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International Immunology Advance Access originally published online on November 29, 2004
International Immunology 2005 17(1):65-72; doi:10.1093/intimm/dxh187
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© 2005 The Japanese Society for Immunology

Requirement of the tyrosines at residues 258 and 270 of MAIR-I in inhibitory effect on degranulation from basophilic leukemia RBL-2H3

Yasushi Okoshi1,2, Satoko Tahara-Hanaoka1, Chigusa Nakahashi1, Shin-ichiro Honda1, Akitomo Miyamoto1, Hiroshi Kojima2, Toshiro Nagasawa2, Kazuko Shibuya1 and Akira Shibuya1

1 Department of Immunology, Institute of Basic Medical Sciences and 2 Department of Clinical and Experimental Hematology, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Japan

Correspondence to: A. Shibuya; E-mail: ashibuya{at}md.tsukuba.ac.jp

Mast cells and basophils express the high affinity receptor for IgE (Fc{varepsilon}RI) and play a central role for IgE-associated immediate hypersensitivity reactions and allergic disorders. Cross-linking of Fc{varepsilon}RI-bound IgE with multivalent antigen initiates the activation of mast cells and basophils, resulting in the degranulation from these cells. We have recently identified a novel inhibitory receptor, myeloid-associated immunoglobulin-like receptor (MAIR)-I, which is expressed on mast cells as well as other myeloid cell lineages. Co-ligation of Fc{varepsilon}RI and MAIR-I inhibits IgE-mediated degranulation from mast cells. However, MAIR-I-mediated signaling pathways involved in the inhibition remain undetermined. Here, we demonstrate that the transfectant of rat basophil leukemia RBL-2H3 expressing wild-type MAIR-I is tyrosine phosphorylated and recruits SHP-1 and SHIP upon cross-linking of MAIR-I. By using RBL-2H3 transfectants expressing variable mutant MAIR-I at Y233, Y258, Y270 and/or Y299, we further demonstrate that both Y258 and Y270, but not Y233 and Y299, were phosphorylated and were essentially required for inhibition of IgE-mediated degranulation from the RBL-2H3 transfectant.

Keywords: Fc{varepsilon}RI, inhibitory signal, ITIM, mast cell

Transmitting editor: T. Takai


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