International Immunology Advance Access originally published online on August 9, 2004
International Immunology 2004 16(9):1305-1313; doi:10.1093/intimm/dxh133
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© 2004 The Japanese Society for Immunology
The TL MHC class Ib molecule has only marginal effects on the activation, survival and trafficking of mouse small intestinal intraepithelial lymphocytes
1 Laboratory of Viral Diseases, NIAID, NIH, Bethesda, MD, USA
2 Laboratoire de Biologie Moléculaire du Gene, INSERM U277, Institut Pasteur, Paris, France
3 Laboratory of Clinical Investigation, NIAID, NIH, Bethesda, MD, USA
Correspondence to: Nathalie Pardigon; E-mail: npardigon{at}niaid.nih.gov
Thymus leukemia antigen (TL) is an MHC class Ib molecule that is highly conserved in rats and mice with no obvious human homolog. TL is expressed in mouse small intestinal epithelial cells and is known to interact with CD8
homodimers, which are expressed by intraepithelial lymphocytes (IELs), some other T cell subsets and some non-T cells such as a subset of dendritic cells. We show here that TL is abundantly expressed on the basolateral surface of mouse small intestinal epithelial cells and that expression is abrogated in ß2m–/– mice but unaffected in TCR–/– mice or CD8 chain–/– mice. We demonstrate that the interaction between TL and CD8 is not necessary for IEL survival in vitro or in vivo and does not modulate IEL trafficking in vivo. TL co-stimulation of -CD3 antibody-activated IELs resulted in modestly enhanced production of IFN-
in one subset of IELs. The lack of effect on IEL survival and trafficking and the modest effect on IFN- production suggest that the functional consequences of TL interaction with CD8 as well as the more general biological role of TL in mucosal immunity remains to be discovered.
Keywords: intraepithelial lymphocytes, mouse small intestine, thymic leukemia ag
Transmitting editor: P. Ohashi
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