International Immunology Advance Access originally published online on March 29, 2004
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
International Immunology, Vol. 16, No. 5, pp. 707-716,
May 2004
© 2004 Japanese Society for Immunology
The role of adjuvant on the regulatory effects of NK cells on B cell responses as revealed by a new model of NK cell deficiency
1 Laboratory of Molecular Pathology, Department of Pathology, UT Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA
Correspondence to: D. Yuan; E-mail: Dorothy.Yuan{at}UTSouthwestern.edu
Transmitting editor: P. Kincade
We have utilized a novel method to generate transgenic mice that are deficient in NK cells. The strategy entails introduction of the H and L chain genes encoding PK136, an antibody shown to be effective in the in vivo elimination of NK cells, into the mouse genome. Since the introduced H chain gene does not contain sequences encoding membrane exons, the transgenic Ig is not expressed on the cell surface, but is secreted by activated B cells. We show that these animals are chronically depleted of NK cells, but not B, T or NKT cells. Therefore, they are compromised in their ability to mediate NK-mediated cytotoxicity. In addition, the deficiency in NK cells reduces the level of switching to various downstream isotypes in response to T-independent type II antigens. However, this reduction is only apparent when antigens are injected in the presence of adjuvant. Since NKT cells are not depleted, the effect cannot be attributed to this subpopulation. These results help to resolve differences in previous findings regarding the role of NK cells in antibody responses.
Keywords: Ficoll, isotype switching, lung clearance, Pneumovax, transgenic
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  P. Jennings and D. Yuan NK Cell Enhancement of Antigen Presentation by B Lymphocytes J. Immunol., March 1, 2009; 182(5): 2879 - 2887. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Nandakumar, S. N. Woolard, D. Yuan, B. T. Rouse, and U. Kumaraguru Natural Killer Cells as Novel Helpers in Anti-Herpes Simplex Virus Immune Response J. Virol., November 1, 2008; 82(21): 10820 - 10831. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Gao, P. Jennings, and D. Yuan Requirements for the natural killer cell-mediated induction of IgG1 and IgG2a expression in B lymphocytes Int. Immunol., May 1, 2008; 20(5): 645 - 657. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Gao, P. Schwartzberg, J. A. Wilder, B. R. Blazar, and D. Yuan B Cell Induction of IL-13 Expression in NK Cells: Role of CD244 and SLAM-Associated Protein. J. Immunol., March 1, 2006; 176(5): 2758 - 2764. [Abstract] [Full Text] [PDF]
|
|