Skip Navigation


International Immunology Advance Access originally published online on April 5, 2004
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
16/5/625    most recent
dxh058v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (5)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Kovalenko, P.
Right arrow Articles by Yamamoto, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kovalenko, P.
Right arrow Articles by Yamamoto, T.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

International Immunology, Vol. 16, No. 5, pp. 625-634, May 2004
© 2004 Japanese Society for Immunology

Fc receptor-mediated accumulation of macrophages in crescentic glomerulonephritis induced by anti-glomerular basement membrane antibody administration in WKY rats

Pavel Kovalenko1, Hidehiko Fujinaka1, Yutaka Yoshida1, Hiroki Kawamura2, Zhenyun Qu1, Adel Galal Ahmed El-Shemi1, Huiping Li1, Asako Matsuki1, Vladimir Bilim1, Eishin Yaoita1, Toru Abo2, Makoto Uchiyama3 and Tadashi Yamamoto1

1 Department of Structural Pathology, Institute of Nephrology, 2 Division of Immunology and Zoology, Department of Infectious Disease Control, Course for Community Disease Control and 3 Division of Pediatrics, Department of Homeostatic Regulation and Development, Course for Biological Functions and Medical Control, Graduate School of Medical and Dental Sciences, Niigata University, Niigata 951-8510, Japan

Correspondence to: T. Yamamoto; E-mail: tdsymmt{at}med.niigata-u.ac.jp
Transmitting editor: K. Okumura

Anti-glomerular basement membrane (GBM) glomerulonephritis induced in WKY rats is characterized by glomerular accumulation of CD8+ T cells and monocytes/macrophages, followed by crescent formation. The mechanism of leukocyte accumulation after antibody binding to GBM is still unclear. To unveil an involvement of Fc{gamma} receptors (Fc{gamma}R) in leukocytes recruitment we examined the expression of Fc{gamma}R in glomeruli and the effects of the administration of F(ab')2 fragment of anti-GBM antibody or Fc{gamma}R blocking on the initiation and progression of this model. A gradual increase of Fc{gamma}R mRNA expression in glomeruli during the time course of disease suggested their significance in the development of glomerulonephritis. Glomerular lesions and proteinuria were induced only in rats injected with intact IgG of anti-GBM antibody, but not with the F(ab')2 fragment. In vivo blocking of Fc{gamma}R by administering heat-aggregated IgG led to the decrease of mRNA expression for all types of Fc{gamma}R (types 1, 2 and 3) and a significant amelioration of glomerulonephritis manifestations. By flow cytometry and immunohistochemistry Fc{gamma}R2-expressing cells in glomeruli were identified as macrophages, but not CD8+ T cells. The expression of Fc{gamma}R1 and 3 was significantly decreased, and that of Fc{gamma}R2 became undetectable in CD8+ T cell-depleted rats. Thus, CD8+ T cells may stimulate Fc{gamma}R expression on macrophages, contributing to their glomerular accumulation and injury. These studies provide direct evidence for a crucial involvement of IgG Fc–Fc{gamma}R interaction in glomerular recruitment of macrophages and following induction of anti-GBM glomerulonephritis in WKY rats.

Keywords: anti-glomerular basement membrane antibody, CD8+ T cell, crescentic glomerulonephritis, Fc{gamma}R, macrophage


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Immunol.Home page
A. Bergtold, A. Gavhane, V. D'Agati, M. Madaio, and R. Clynes
FcR-Bearing Myeloid Cells Are Responsible for Triggering Murine Lupus Nephritis
J. Immunol., November 15, 2006; 177(10): 7287 - 7295.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.