Skip Navigation


International Immunology Advance Access originally published online on September 20, 2004
International Immunology 2004 16(11):1561-1571; doi:10.1093/intimm/dxh157
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
16/11/1561    most recent
dxh157v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (7)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Cloosen, S.
Right arrow Articles by Bos, G. M. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cloosen, S.
Right arrow Articles by Bos, G. M. J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?


© 2004 The Japanese Society for Immunology

Mucin-1 is expressed on dendritic cells, both in vitro and in vivo

Silvie Cloosen, Marco Thio, Ariane Vanclée, Ellen B. M. van Leeuwen, Birgit L. M. G. Senden-Gijsbers, Ellis B. H. Oving, Wilfred T. V. Germeraad and Gerard M. J. Bos

Department of Internal Medicine, Division of Hemato-Oncology, University Hospital Maastricht, PO Box 5800, 6202 AZ Maastricht, The Netherlands

Correspondence to: S. Cloosen; E-mail: S.Cloosen{at}immuno.unimaas.nl

Dendritic cells (DCs) are the best professional antigen-presenting cells to stimulate cytotoxic as well as T helper cells and are therefore appropriate candidates for establishing immunotherapy. The concept of our vaccination program is to introduce the tumor-associated antigen mucin-1 (MUC1) into DCs. Analysis of immature and mature DCs—before transducing the antigen MUC1—already demonstrated expression of MUC1 on in vitro monocyte-derived DCs upon maturation. Different culture methods as well as maturation cocktails showed similar results concerning the upregulation of MUC1 expression. Furthermore, we studied the expression of MUC1 on DCs in vivo. No MUC1 expression was found on blood DCs, or on thymic or tonsil DCs. On the other hand, synovial fluid from patients with arthritis contained DCs that were found to express MUC1. This study shows for the first time that the tumor-associated antigen MUC1 is expressed on in vivo DCs. We further show that MUC1 is also expressed on in vitro cultured bone marrow-derived DCs of human MUC1 transgenic mice, supporting the relevance of this mouse model to the human situation. The observation that MUC1 is present on in vivo DCs suggests a functional role, but this physiological function remains to be elucidated.

Keywords: human, immunotherapy, synovial fluid, transgenic mice

Transmitting editor: K. Inaba


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Cancer Res.Home page
S. Cloosen, J. Arnold, M. Thio, G. M.J. Bos, B. Kyewski, and W. T.V. Germeraad
Expression of Tumor-Associated Differentiation Antigens, MUC1 Glycoforms and CEA, in Human Thymic Epithelial Cells: Implications for Self-Tolerance and Tumor Therapy
Cancer Res., April 15, 2007; 67(8): 3919 - 3926.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.