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International Immunology, Vol. 15, No. 4, pp. 515-524, April 2003
© 2003 Japanese Society for Immunology

Developmental stages of myeloid dendritic cells in mouse bone marrow

Tatjana Nikolic1, Marella F. T. R. de Bruijn1,3, Manfred B. Lutz2 and Pieter J. M. Leenen1

1 Department of Immunology, Erasmus MC, University Medical Center, PO Box 1738, 3000 DR Rotterdam, The Netherlands 2 Department of Dermatology, University of Erlangen, D-91052 Erlangen, Germany 3 Present address: Weatherall Institute of Molecular Medicine, University of Oxford, Oxford OX3 9DC, UK

The first two authors contributed equally to this work
Correspondence to: T. Nikolic; E-mail: t.nikolic{at}erasmusmc
Transmitting editor: P. W. Kincade

The lineage relationship of dendritic cells (DC) with other hematopoietic cell types has been studied extensively, resulting in the identification of different bone marrow (BM) progenitors that give rise to distinct DC types. However, the identity of the different maturation stages of DC precursors in the BM remains unclear. In this study we define the in vivo developmental steps of the myeloid DC lineage in mouse BM. To this end, BM cells were separated according to their expression of CD31 (ER-MP12), Ly-6C (ER-MP20) and ER-MP58 antigens, and stimulated to develop into myeloid DC, using granulocyte macrophage colony stimulating factor as a specific growth factor. DC developed from three BM subpopulations: ER-MP12hi/20– (early blast cells), ER-MP12+/20+ (myeloid blasts) and ER-MP12–/20hi (monocytes). The kinetic and phenotypic features of DC developing in vitro indicate that the three populations represent successive maturation stages of myeloid DC precursors. Within the earliest ER-MP12hi/20– population, DC precursors exclusively occurred in the myeloid-restricted ER-MP58hi subset. By using switch cultures, we show that these BM precursor subpopulations, when stimulated to develop into macrophages using macrophage colony stimulating factor, retain the ability to develop into myeloid DC until advanced stages of maturation. Together, these findings support a common ER-MP12/20-defined differentiation pathway for both macrophages and myeloid DC throughout their BM development.

Keywords: bone marrow, dendritic cells, myeloid development


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