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International Immunology, Vol. 15, No. 3, pp. 411-416, March 2003
© 2003 Japanese Society for Immunology

Expression cloning and function of the rat NK activating and inhibitory receptors NKR-P1A and -P1B

Jennifer Li1,2, Brian A. Rabinovich1,2, Rose Hurren1,2, John Shannon1,2 and Richard G. Miller1,2

Departments of 1 Medical Biophysics and 2 Immunology, University of Toronto and the Ontario Cancer Institute, Toronto, Ontario M5G 2M9, Canada

Correspondence to: R. G. Miller, Department of Medical Biophysics, Ontario Cancer Institute, Room 9-305, 610 University Avenue, Toronto, Ontario, M5G 2M9, Canada. E-mail: miller{at}oci.utoronto.ca
Transmitting editor: W. M. Yokoyama

We have characterized the rat NK receptors NKR-P1A and -P1B. A cDNA library was constructed from the rat NK cell line, RNK-16. Using the pMX retroviral cloning system, the library was expressed in the human NK cell line, YTSeco, and cells staining with the anti-rat mAb 10/78 identified, FACS sorted and cloned. Two genes, corresponding to rat NK receptors NKR-P1A and -P1B, were identified. YTSeco clones expressing either NKR-P1A or -P1B were functionally tested using 51Cr-release redirected lysis assays and calcium flux experiments. This demonstrated that NKR-P1A functions as an activation receptor, as previously shown, and that NKR-P1B functions as an inhibitory receptor, as predicted by the presence of an immunoreceptor tyrosine-based inhibition motif. Although annotated as NKR-P1A specific, we found that mAb 10/78 stained YTSeco clones expressing NKR-P1A or -P1B equally well, as did the mAb 3.2.3 used for the original cloning of rat NKR-P1A.

Keywords: calcium flux, cytotoxicity, lymphokine activated killer cell


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