International Immunology, Vol. 15, No. 12, pp. 1431-1440,
December 2003
© 2003 Japanese Society for Immunology
FEATURED ARTICLE OF THE MONTH |
Impaired signaling via the high-affinity IgE receptor in WiskottAldrich syndrome protein-deficient mast cells
1 Division of Immunology, Childrens Hospital, 2 Division of Gastroenterology, Massachusetts General Hospital, 3 Division of Hematology, Brigham and Womens Hospital, and 4 Howard Hughes Medical Institute, Childrens Hospital, Harvard Medical School, Boston, MA 02115, USA 5 Present address: Department of Cell Biology and Anatomy, University of Arizona, Tucson, AZ 85724-5030, USA
Correspondence to: R. S. Geha; E-mail: raif.geha{at}tch.harvard.edu
Transmitting editor: S. J. Galli
WiskottAldrich syndrome protein (WASP) is the product of the gene deficient in boys with X-linked WiskottAldrich syndrome. We assessed the role of WASP in signaling through the high-affinity IgE receptor (Fc
RI) using WASP-deficient mice. IgE-dependent degranulation and cytokine secretion were markedly diminished in bone marrow-derived mast cells from WASP-deficient mice. Upstream signaling events that include Fc
RI-triggered total protein tyrosine phosphorylation, and protein tyrosine phosphorylation of Fc
RIß and Syk were not affected by WASP deficiency. However, tyrosine phosphorylation of phospholipase C
and Ca2+ mobilization were diminished. IgE-dependent activation of c-Jun N-terminal kinase, cell spreading and redistribution of cellular F-actin in mast cells were reduced in the absence of WASP. We conclude that WASP regulates Fc
RI-mediated granule exocytosis, cytokine production and cytoskeletal changes in mast cells.
Keywords: allergy and immunology, cell degranulation, receptor-mediated signal transduction, WiskottAldrich syndrome
![]()
CiteULike
Connotea
Del.icio.us What's this?
This article has been cited by other articles:
![]() |
W. Huang, H. D. Ochs, B. Dupont, and Y. M. Vyas The Wiskott-Aldrich Syndrome Protein Regulates Nuclear Translocation of NFAT2 and NF-{kappa}B (RelA) Independently of Its Role in Filamentous Actin Polymerization and Actin Cytoskeletal Rearrangement J. Immunol., March 1, 2005; 174(5): 2602 - 2611. [Abstract] [Full Text] [PDF] |
||||
![]() |
S. Burns, G. O. Cory, W. Vainchenker, and A. J. Thrasher Mechanisms of WASp-mediated hematologic and immunologic disease Blood, December 1, 2004; 104(12): 3454 - 3462. [Abstract] [Full Text] [PDF] |
||||

