International Immunology, Vol. 15, No. 11, pp. 1301-1307,
November 2003
© 2003 Japanese Society for Immunology
Recognition mechanism of non-peptide antigens by human 
T cells
1 Department of Immunology and Cell Biology, Graduate School of Biostudies and 2 Department of Pediatrics, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, Japan 3 Laboratory of Food Quality Design and Development, Graduate School of Agriculture, Kyoto University, Kyoto 611-0011, Japan 4 PRESTO, JST, Kyoto, Japan
Correspondence to: N. Minato; E-mail: minato{at}imm.med.kyoto-u.ac.jp
Transmitting editor: S. Koyasu
The majority of 
T cells in adult human blood exhibit V2/V2-TCR and specifically respond to various kinds of non-peptide antigens. In this study, we comparatively analyzed the CDR3 repertoires of V2- and V2- chain genes in the adult and cord blood. It was confirmed that the vast majority of adult T cells exhibited V2- chains bearing a J1.2 segment with no or short N-region and V2- chains with a conserved hydrophobic residue (leucine, valine or isoleucine) at position 97 encoded by N-region of V/J junction (L97). The cord blood cells stimulated with pyrophosphomonoester antigen in vitro showed preferential expansion of the T cells expressing V2- and V2-TCR chains with these structural features as compared with those stimulated with a polyclonal mitogen phytohemagglutinin. TCR gene transfer studies indicated that alanine substitution of lysine at position 108 in J1.2 (K108) or L97 abrogated the responsiveness of V2/V2-TCR to all kinds of the non-peptide antigens without affecting the response to anti-CD3 antibody. Furthermore, alanine substitution of arginine at position 51 in V2 segment (R51) adjacent to K108 in the V2/V2- TCR also abolished the antigen responsiveness. These results strongly suggested that a hydrophobic and two cationic residues (L97, K108 and R51) clustered in a particular topology at the surface edge of the pocket structure of V2/V2- TCR played essential roles in the recognition of non-peptide antigens.
Keywords: TCR, antigen recognition, antigenic selection, infection immunity, pyrophosphomonoester antigen
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