Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (14)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Salio, M.
Right arrow Articles by Cerundolo, V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Salio, M.
Right arrow Articles by Cerundolo, V.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

International Immunology, Vol. 15, No. 10, pp. 1265-1273, October 2003
© 2003 Japanese Society for Immunology

Efficient priming of antigen-specific cytotoxic T lymphocytes by human cord blood dendritic cells

Mariolina Salio1, Nicolas Dulphy1, Joelle Renneson1, Mark Herbert2, Andrew McMichael2, Arnaud Marchant2 and Vincenzo Cerundolo1

1 Cancer Research UK Tumour Immunology Unit and 2 Human Immunology Unit, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, OX3 9DS, Oxford, UK

Correspondence to: M. Salio; E-mail: msalio{at}molbiol.ox.ac.uk or V. Cerundolo; E-mail: vincenzo.cerundolo{at}imm.ox.ac.uk
Transmitting editor: E. Simpson

Previous studies have suggested that defective immune responses in early life may be related to the immaturity of neonatal antigen-presenting cells. To test this hypothesis, we assessed the capacity of neonatal dendritic cells (DC) to prime and polarize in vitro human naive antigen-specific T cells. We report that mature cord blood DC efficiently prime an oligoclonal population of antigen-specific CD8 T cells, capable of cytolytic activity and IFN-{gamma} secretion. In contrast, cells primed by immature cord blood DC do not acquire cytolytic activity and secrete lower amounts of IFN-{gamma}. Upon priming by either immature or mature DC, neonatal T cells acquire markers of activation and differentiation towards effector-memory cells. Our results demonstrate that, if appropriately activated, neonatal DC can prime efficient cytotoxic T lymphocyte (CTL) responses. Furthermore, these findings have important implications for the development of vaccine strategies in early life and for the reconstitution of a functional CTL repertoire after bone marrow transplantation.

Keywords: neonatal immunity, T cell polarization, tetramer


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 CVIHome page
M. Motamedi, S. Arab, S. M. Moazzeni, M. Khamis Abadi, and J. Hadjati
Improvement of a Dendritic Cell-Based Therapeutic Cancer Vaccine with Components of Toxoplasma gondii
Clin. Vaccine Immunol., October 1, 2009; 16(10): 1393 - 1398.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
B. A. Colleton, X.-L. Huang, N. M. Melhem, Z. Fan, L. Borowski, G. Rappocciolo, and C. R. Rinaldo
Primary Human Immunodeficiency Virus Type 1-Specific CD8+ T-Cell Responses Induced by Myeloid Dendritic Cells
J. Virol., June 15, 2009; 83(12): 6288 - 6299.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
C. Schuster, C. Vaculik, C. Fiala, S. Meindl, O. Brandt, M. Imhof, G. Stingl, W. Eppel, and A. Elbe-Burger
HLA-DR+ leukocytes acquire CD1 antigens in embryonic and fetal human skin and contain functional antigen-presenting cells
J. Exp. Med., January 16, 2009; 206(1): 169 - 181.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
L. M'Rabet, A. P. Vos, G. Boehm, and J. Garssen
Breast-Feeding and Its Role in Early Development of the Immune System in Infants: Consequences for Health Later in Life
J. Nutr., September 1, 2008; 138(9): 1782S - 1790S.
[Full Text] [PDF]

Home page
 Int ImmunolHome page
N. C. Matthews, U. F. Power, and D. J. Reen
Neonatal human autologous dendritic cells pulsed with recombinant protein antigen prime the generation of non-polarized CD4 T-cell effectors
Int. Immunol., June 1, 2007; 19(6): 703 - 712.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
B. Mommaas, J. A. Stegehuis-Kamp, A. G. van Halteren, M. Kester, J. Enczmann, P. Wernet, G. Kogler, T. Mutis, A. Brand, and E. Goulmy
Cord blood comprises antigen-experienced T cells specific for maternal minor histocompatibility antigen HA-1
Blood, February 15, 2005; 105(4): 1823 - 1827.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. H. Qureshi, K. M. Empey, and B. A. Garvy
Modulation of Proinflammatory Responses to Pneumocystis carinii f. sp. muris in Neonatal Mice by Granulocyte-Macrophage Colony-Stimulating Factor and IL-4: Role of APCs
J. Immunol., January 1, 2005; 174(1): 441 - 448.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. Regner, X. Martinez, E. Belnoue, C.-M. Sun, F. Boisgerault, P.-H. Lambert, C. Leclerc, and C.-A. Siegrist
Partial Activation of Neonatal CD11c+ Dendritic Cells and Induction of Adult-Like CD8+ Cytotoxic T Cell Responses by Synthetic Microspheres
J. Immunol., August 15, 2004; 173(4): 2669 - 2674.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.