International Immunology, Vol. 14, No. 8, pp. 883-892,
August 2002
© 2002 Japanese Society for Immunology
Chemotaxis of human tonsil B lymphocytes to CC chemokine receptor (CCR) 1, CCR2 and CCR4 ligands is restricted to non-germinal center cells
1 Laboratory of Oncology, G. Gaslini Institute, Largo G. Gaslini 5, 16148 Genova, Italy 2 Department of Internal Medicine, University of Genova, Genova, Italy 3 Department of Immunology, Mario Negri Institute, Milano, Italy 4 Division of Otolaryngology, G. Gaslini Institute, Genova, Italy 5 Department of Genetics, Biology and Biochemistry, University of Torino, Torino, Italy
Correspondence to: A. Corcione; E-mail: annacorcione{at}ospedale-gaslini.ge.it
Transmitting editor: L. Moretta
We have investigated the effects of nine CC chemokines, i.e. macrophage inflammatory protein (MIP)-1
/CCL3, MIP-1ß/CCL4, MIP-3/CCL20, MIP-5/CCL15, monocyte chemotactic protein (MCP)-1/CCL2, MCP-2/CCL8, MCP-3/CCL7, eotaxin/CCL11 and macrophage-derived chemokine (MDC)/CCL22 on the locomotion of human tonsil B lymphocytes and their subsets. Upon isolation, B cells were poorly responsive, but, following short-term culture, they displayed statistically significant chemotactic responses (P < 0.001) to MIP-1, MIP-5, MCP-1, MCP-2, MCP-3 and MDC. CC chemokine receptor (CCR) 1 to CCR6 were up-regulated after culture. MIP-1ß, MIP-3 and eotaxin did not stimulate B cell migration. Scattered information is available on B cell subset responses to chemokines. Therefore, we investigated the effects of MIP-1, MIP-5, MCP-1, MCP-2, MCP-3 and MDC on the in vitro locomotion of non-germinal center (GC) (CD38–) and GC (CD38+) B cells. All chemokines enhanced significantly (P < 0.001) the migration of the former, but not of the latter, cells. CCR1, CCR2 and CCR4 were detected by flow cytometry on non-GC (i.e. naive and memory) B cells, whereas they were absent (CCR1 and CCR2) or poorly expressed (CCR4) on GC B cells.
Keywords: B cell subsets, chemokines, chemokine receptors, locomotion
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