International Immunology, Vol. 14, No. 6, pp. 585-597,
June 2002
© 2002 Japanese Society for Immunology
CD6: expression during development, apoptosis and selection of human and mouse thymocytes
1 Case Western Reserve University School of Medicine and Rainbow Babies and Children’s Hospital/University Hospitals of Cleveland, Cleveland, OH 44106, USA 2 University of Michigan Medical Center, Ann Arbor, MI 48109, USA 3 Stanford University Medical Center, Stanford, CA 94305, USA 4 Present address: Mt Sinai–NYU Health System, New York, NY 10029, USA
Correspondence to: N. G. Singer, 504 Rainbow Babies and Children’s Hospital, 11100 Euclid Avenue, Cleveland OH 44106, USA. E-mail: ngs{at}po.cwru.edu
CD6, a 130-kDa surface glycoprotein, is expressed primarily on cells of T lineage. A co-stimulatory role for CD6 in mature T cells has been shown, but the function of CD6 during thymocyte development is unknown. Since CD6 ligands are expressed on thymic epithelium, their interactions with CD6 could be important in thymic selection. In this report we show that CD6 is developmentally regulated in human and mouse thymocytes, and further demonstrate that increase in the level of CD6 expression correlates with expression of the selection marker CD69. We also show that activation via CD2 induces CD6 expression on mature human thymocytes and on a subset of immature human thymocytes that are resistant to apoptosis. In human and mouse thymocytes that express heterogeneous TCR, CD6 increases occur as double-positive thymocytes are selected to a single-positive stage. In contrast, in thymocytes from TCR transgenic mice, CD6 is barely increased following selection, suggesting that as functional avidity increases, requirements for CD6-dependent co-stimulation decrease. Taken together, these results indicate that during thymic development CD6-dependent signals may contribute both to thymocyte survival, and to the overall functional avidity of selection in both man and mouse.
Keywords: apoptosis, comparative immunology, co-stimulation, T cells, thymus
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  S.-C. Choi, K. D. Kim, J.-T. Kim, J. W. Kim, H. G. Lee, J.-M. Kim, Y.-S. Jang, D.-Y. Yoon, K. I. Kim, Y. Yang, et al. Expression of human NDRG2 by myeloid dendritic cells inhibits down-regulation of activated leukocyte cell adhesion molecule (ALCAM) and contributes to maintenance of T cell stimulatory activity J. Leukoc. Biol., January 1, 2008; 83(1): 89 - 98. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. G. Kasler and E. Verdin Histone Deacetylase 7 Functions as a Key Regulator of Genes Involved in both Positive and Negative Selection of Thymocytes Mol. Cell. Biol., July 15, 2007; 27(14): 5184 - 5200. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. A. Castro, M. I. Oliveira, R. J. Nunes, S. Fabre, R. Barbosa, A. Peixoto, M. H. Brown, J. R. Parnes, G. Bismuth, A. Moreira, et al. Extracellular Isoforms of CD6 Generated by Alternative Splicing Regulate Targeting of CD6 to the Immunological Synapse J. Immunol., April 1, 2007; 178(7): 4351 - 4361. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. J. Hassan, S. J. Simmonds, N. G. Clarkson, S. Hanrahan, M. J. Puklavec, M. Bomb, A. N. Barclay, and M. H. Brown CD6 Regulates T-Cell Responses through Activation-Dependent Recruitment of the Positive Regulator SLP-76. Mol. Cell. Biol., September 1, 2006; 26(17): 6727 - 6738. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Ibanez, M.-R. Sarrias, M. Farnos, I. Gimferrer, C. Serra-Pages, J. Vives, and F. Lozano Mitogen-Activated Protein Kinase Pathway Activation by the CD6 Lymphocyte Surface Receptor J. Immunol., July 15, 2006; 177(2): 1152 - 1159. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Gimferrer, A. Ibanez, M. Farnos, M.-R. Sarrias, R. Fenutria, S. Rosello, P. Zimmermann, G. David, J. Vives, C. Serra-Pages, et al. The Lymphocyte Receptor CD6 Interacts with Syntenin-1, a Scaffolding Protein Containing PDZ Domains J. Immunol., August 1, 2005; 175(3): 1406 - 1414. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Niederberger, L. K. Buehler, J. Ampudia, and N. R. J. Gascoigne Thymocyte stimulation by anti-TCR-{beta}, but not by anti-TCR-{alpha}, leads to induction of developmental transcription program J. Leukoc. Biol., May 1, 2005; 77(5): 830 - 841. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. K. Saifullah, D. A. Fox, S. Sarkar, S. M. A. Abidi, J. Endres, J. Piktel, T. M. Haqqi, and N. G. Singer Expression and Characterization of a Novel CD6 Ligand in Cells Derived from Joint and Epithelial Tissues J. Immunol., November 15, 2004; 173(10): 6125 - 6133. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-C. Wang, D. Montufar-Solis, B.-B. Teng, and J. R. Klein Maximum Immunobioactivity of Murine Small Intestinal Intraepithelial Lymphocytes Resides in a Subpopulation of CD43+ T Cells J. Immunol., November 15, 2004; 173(10): 6294 - 6302. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Gimferrer, M. Calvo, M. Mittelbrunn, M. Farnos, M. R. Sarrias, C. Enrich, J. Vives, F. Sanchez-Madrid, and F. Lozano Relevance of CD6-Mediated Interactions in T Cell Activation and Proliferation J. Immunol., August 15, 2004; 173(4): 2262 - 2270. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Gimferrer, M. Farnos, M. Calvo, M. Mittelbrunn, C. Enrich, F. Sanchez-Madrid, J. Vives, and F. Lozano The Accessory Molecules CD5 and CD6 Associate on the Membrane of Lymphoid T Cells J. Biol. Chem., February 28, 2003; 278(10): 8564 - 8571. [Abstract] [Full Text] [PDF]
|
|