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International Immunology, Vol. 14, No. 3, 259-266, March 2002
© 2002 Japanese Society for Immunology

Reversible CD8 expression induced by common cytokine receptor {gamma} chain-dependent cytokines in a cloned CD4+ Th1 cell line

Cheung-Seog Park, Yi-Fu Yang, Xu-Yu Zhou, Kazuhito Toyooka, Yumi Yashiro-Ohtani, Woong-Ryeon Park, Michio Tomura, Xu-Guang Tai, Toshiyuki Hamaoka and Hiromi Fujiwara

Department of Oncology, Osaka University Graduate School of Medicine, 2-2 Yamada-oka, Suita, Osaka 565-0871, Japan

Correspondence to: H. Fujiwara; E-mail: hf{at}oncogene.med.osaka-u.ac.jp

T cells that are intrathymically lineage committed are believed to maintain their CD4 or CD8 co-receptor expression. Here, we investigated whether intrathymic lineage commitment involves irreversible genetic modification or whether co-receptor expression can be reprogrammed depending on external stimuli. The CD4+ Th1 clone 2D6 established from splenic T cells as an IL-12-dependent line survived in culture with IL-2, IL-7 or IL-15 alone. Surprisingly, CD8 expression occurred in 2D6 cells upon replacement of IL-12 with any one of the three cytokines that stimulate the common cytokine receptor {gamma} chain, yielding CD4+CD8+ 2D6 cells. CD8 expression declined when IL-2 was replaced with IL-12 and CD8 induction was inhibited when IL-12 was included in IL-2 or IL-7 culture. Our observations show that even a lineage-committed mature T cell can be reprogrammed for co-receptor expression in response to particular external stimuli.

Keywords: CD4, CD8, cell surface molecules, cytokine, IL-2, IL-12


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