International Immunology, Vol. 14, No. 3, 241-247,
March 2002
© 2002 Japanese Society for Immunology
Modeling the presentation of C3d-coated antigen by B lymphocytes: enhancement by CR1/2–BCR co-ligation is selective for the co-ligating antigen
1 Department of Immunology, Loránd Eötvös University, Pázmány Péter s. 1/C, Budapest, Hungary
2 Research Group of the Hungarian Academy of Sciences, Budapest, Hungary
3 Center of Immunology, Bucharest, Romania
Correspondence to: A. Erdei, Department of Immunology, Loránd Eötvös University, Pázmány Péter s. 1/C, Budapest, Hungary. E-mail: anna.erdai{at}freemail.hu
We have used a set of single-chain variable fragment antibodies (sc) genetically fused with an influenza hemagglutinin-derived peptide as a means to investigate the role of CR1 and CR2 in antigen presentation by B cells. When incubated with the B cell lymphoma 2PK3, peptide-containing sc specific for either CR1 or CR1/2 mediated activation of the hemagglutinin peptide-specific T cell line IP-12-7, as assessed by IL-2 production. Efficient presentation was dependent on the binding of the constructs to CR1/2, implying that receptor-mediated endocytosis is responsible for the effect. Cross-linkage of CR1/2 or CD19 by mAb did not increase the extent of T cell activation. However, when CR1/2 was co-ligated with the BCR—using either polyclonal goat anti-mouse IgG or recombinant protein LA—the antigen concentration required to activate T cells decreased by two orders of magnitude. Moreover, this enhancement was selective for the antigen included in these complexes and did not affect the presentation of a free peptide or of antigen bound to CR1/2 excluded from the complexes. These results suggest that B cells may bind various C3d-coated antigens at a time, but only the one which reacts with the BCR will be processed with high efficiency. This mechanism may ensure the specificity of cognate T cell help.
Keywords: antigen presentation, B lymphocytes, complement
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  S. L. Brown, D. V. Barrault, A. Phythian-Adams, and A. M. Knight Lack of induced co-stimulation as a result of complement receptor 2 (CR2) ligation on mouse splenic B cells Int. Immunol., January 1, 2006; 18(1): 69 - 78. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Li, H. Patel, C. A. Farrar, R. E.G. Hargreaves, S. H. Sacks, and W. Zhou Complement Activation Regulates the Capacity of Proximal Tubular Epithelial Cell to Stimulate Alloreactive T Cell Response J. Am. Soc. Nephrol., September 1, 2004; 15(9): 2414 - 2422. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. V. Barrault and A. M. Knight Distinct Sequences in the Cytoplasmic Domain of Complement Receptor 2 Are Involved in Antigen Internalization and Presentation J. Immunol., March 15, 2004; 172(6): 3509 - 3517. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Greicius, L. Westerberg, E. J. Davey, E. Buentke, A. Scheynius, J. Thyberg, and E. Severinson Microvilli structures on B lymphocytes: inducible functional domains? Int. Immunol., February 1, 2004; 16(2): 353 - 364. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-B. Villiers, P. N. Marche, and C. L. Villiers Improvement of long-lasting response and antibody affinity by the complexation of antigen with complement C3b Int. Immunol., January 1, 2003; 15(1): 91 - 95. [Abstract] [Full Text] [PDF]
|
|