Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (5)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Guo, Y.
Right arrow Articles by Zheng, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Guo, Y.
Right arrow Articles by Zheng, P.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

International Immunology, Vol. 14, No. 2, 189-200, February 2002
© 2002 Japanese Society for Immunology

Cis elements for transporter associated with antigen-processing-2 transcription: two new promoters and an essential role of the IFN response factor binding element in IFN-{gamma}-mediated activation of the transcription initiator

Yong Guo2, Tianyu Yang, Xingluo Liu, Shengli Lu, Jing Wen, Joan E. Durbin1, Yang Liu and Pan Zheng

Department of Pathology and Comprehensive Cancer Center, Ohio State University Medical Center, 129 Hamilton Hall, 1645 Neil Avenue, Columbus, OH 43210, USA
1 Department of Pediatrics, Columbus Children's Hospital, Ohio State University, Columbus, OH 43205, USA
2 Present address: Hoechst Marion Rousse, Inc., CNS-Molecular Biology, Bridgewater, NJ 08807, USA

Correspondence to: P. Zheng; E-mail: zheng-1{at}medctr.osu.edu

Expression of cell surface MHC class I:peptide complex requires coordinated expression of multiple genes such as MHC class I heavy chain, ß2-microglobulin (ß2m), transporters associated with antigen-processing (TAP)-1 and TAP-2, and proteosomal components low-molecular weight polypeptide (LMP)-2 and LMP-7. All of these genes are expressed at defined and distinct levels in normal tissues, and are inducible by IFN-{gamma}. While the cis elements involved in transcription of the MHC class I heavy chain, ß2m, TAP-1 and LMP-2 have been analyzed extensively, those for TAP-2 and LMP-7 have not been well studied. Here we systematically analyzed the cis elements for TAP-2 transcription. We found at least two independent elements that are sufficient to activate transcription of a reporter gene. One (hereby called TAP-2 P1) is located 5' to the TAP-2 exon 1, while the other (hereby called TAP-2 P2) is a transcription initiator residing in intron 1. Analysis of the 5' sequence of TAP-2 mRNA indicates that both promoters are active. Moreover, while the TAP-2 promoter region contains cis elements that can mediate TAP-2 induction by IFN-{gamma}, such as {gamma}-activation site and IFN response factor binding element (IRFE), only the IRFE is required for IFN-{gamma} induction of TAP-2 promoter in vitro. The IRFE appears to work as an enhancer for the initiator (P2). Together with another promoter recently identified by others, TAP-2 therefore has three independent promoters that can be differentially regulated.

Keywords: antigen presentation genes, IFN-{gamma} activation, transporters associated with antigen-processing-2, transcriptional regulation

Transmitting editor: R. A. Flavell


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
N. Sizemore, A. Agarwal, K. Das, N. Lerner, M. Sulak, S. Rani, R. Ransohoff, D. Shultz, and G. R. Stark
Inhibitor of {kappa}B kinase is required to activate a subset of interferon {gamma}-stimulated genes
PNAS, May 25, 2004; 101(21): 7994 - 7998.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.