International Immunology, Vol. 14, No. 2, 157-166,
February 2002
© 2002 Japanese Society for Immunology
CD23 defines two distinct subsets of immature B cells which differ in their responses to T cell help signals
Division of Rheumatology, Department of Medicine, and
1 Department of Pathology and Laboratory Medicine, 311 BRB II/III, 421 Curie Boulevard, University of Pennsylvania, PA 19104, USA
2 The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA
Correspondence to: J. G. Monroe; E-mail: monroej{at}mail.med.upenn.edu
Transitional immature B cells undergo apoptosis and fail to proliferate in response to BCR cross-linking, thus representing a target for negative selection of potentially autoreactive B cells in vivo. In agreement with recent reports, transitional B cells were divided into developmentally contiguous subsets based on their surface expression of CD23. When transferred, CD23+ transitional B cells readily localized to the splenic follicles and the outer PALS. Compared with CD23- transitional B cells, CD23+ transitional B cells proliferated more vigorously and were rescued from BCR-induced apoptosis to a greater degree, by T cell help signals. However, both CD23- and CD23+ transitional B cells failed to up-regulate CD86 (B7-2) in response to BCR ligation. These findings demonstrate that phenotypically defined subsets within the transitional B cell population are functionally distinct. Specifically, responsiveness to T cell help is a late acquisition corresponding to the stage when the B cells gain access to peripheral compartments enriched in antigen and activated T cells. The failure of transitional B cells to up-regulate CD86 to BCR-mediated stimulation suggests a unique interaction between transitional B cells and T cells with implications for tolerance in the T cell compartment.
Keywords: B cell subsets, B lymphocytes, cell trafficking, CD23, T cell-B cell collaboration
Transmitting editor: C. J. Paige
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