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International Immunology, Vol. 14, No. 12, pp. 1407-1414, December 2002
© 2002 Japanese Society for Immunology

Engagement of Na,K-ATPase ß3 subunit by a specific mAb suppresses T and B lymphocyte activation

Sawitree Chiampanichayakul1,2,3, Andreas Szekeres3, Panida Khunkaewla1, Seangduen Moonsom1, Vladimir Leksa3, Karel Drbal3, Gerhard J. Zlabinger5, Renate Hofer-Warbinek4, Hannes Stockinger3 and Watchara Kasinrerk1

1 Department of Clinical Immunology, Faculty of Associated Medical Sciences and 2 Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand 3 Institute of Immunology–Vienna International Research Cooperation Center at NFI, and 4 Institute of Vascular Biology and Thrombosis Research, University of Vienna, Brunner Strasse 59, 1235 Vienna, Austria 5 Institute of Immunology, University of Vienna, Borschkegasse 8a, 1090 Vienna, Austria

Correspondence to: W. Kasinrerk; E-mail: watchara{at}chiangmai.ac.th
Transmitting editor: I. Pecht

In order to identify new molecules involved in regulation of T cell proliferation, we generated various mAb by immunization of mice with the T cell line Molt4. We found one mAb (termed P-3E10) that down-regulated the in vitro T cell proliferation induced by CD3-specific OKT3 mAb. The P-3E10 mAb was also able to inhibit IFN-{gamma}, IL-2, IL-4 and IL-10 production of OKT3-activated T cells. The antigen recognized by P-3E10 mAb is broadly expressed on all hematopoietic as well as on all non-hematopoietic cell lines tested so far. Within peripheral blood leukocytes, the P-3E10 antigen was detected on lymphocytes, monocytes and granulocytes. Human umbilical vein endothelial cells (HUVEC) also scored positively. By evaluating the effect of P-3E10 mAb on these cell types we found that it also inhibited anti-IgM-induced B cell proliferation. However, it did not block growth factor-mediated proliferation of HUVEC, and spontaneous proliferation of SupT-1, Jurkat, Molt4 and U937 cell lines. Moreover, it did not influence phagocytosis of human blood monocytes and granulocytes. Biochemical analysis revealed that the P-3E10 antigen is a protein with a mol. wt of 45–50 kDa under non-reducing and 50–55 kDa under reducing conditions. By using a retroviral cloning system, the P-3E10 antigen was cloned. Sequence analysis revealed the P-3E10 antigen to be identical to the ß3 subunit of the Na,K-ATPase.

Keywords: lymphocyte inhibition, mAb, Na,K-ATPase ß3 subunit, retroviral cloning system


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