International Immunology, Vol. 14, No. 11, pp. 1351-1356,
November 2002
© 2002 Japanese Society for Immunology
IL-22, in contrast to IL-10, does not induce Ig production, due to absence of a functional IL-22 receptor on activated human B cells
1 INSRM U454, CHU Arnaud de Villeneuve, 371, Avenue Doyen Gaston Giraud, 34295 Montpellier Cedex 5, France 2 IBMIG, CNRS FRE 2224, 40 Avenue du Recteur Pineau, 86022 Poitiers Cedex, France 3 Institut Génétique Moléculaire, UMR 5535, 1919 Route de Mende, 34033 Montpellier Cedex 1, Montpellier, France
The first two authors contributed equally to this work
Correspondence to: H. Yssel; E-mail: yssel{at}montp.inserm.fr
Transmitting editor: J. Borst
IL-22 is an IL-10 homologue that binds to and signals via the class II cytokine receptor (R) heterodimer IL-22RA1/CFR2-4 (IL-10R2), the latter chain being part of the IL-10R complex. Here, we report that, despite its structural similarity with IL-10, as well as its use of the common IL-10R2 chain, IL-22, in contrast to IL-10, is unable to induce Ig production by activated human B cells. Whereas culture of anti-CD40 mAb-stimulated splenic or tonsillar B cells in the presence of rIL-10 resulted in the production of IgG, IgG1, IgG3 and IgA, rIL-22, at concentrations ranging from 4 to 100 ng/ml, did not induce the production of any of these isotypes. Moreover, unlike rIL-10 which enhanced rIL-4-induced IgG4 and IgE production, rIL-22 was ineffective. Although activated B cells expressed transcripts for a soluble IL-22-binding protein (IL-22RA2), no mRNA for a transmembrane IL-22R (IL-22RA1) could be detected. The latter result was confirmed by the demonstration that rIL-22 failed to induce activation of STAT-3 and -5 in resting or activated B cells. Together, these data show that IL-22, in contrast to its homologue IL-10, is not involved in the immunological activity of B cells, which is due to the absence of a functional IL-22R at the surface of these cells.
Keywords: B cell, ELISA, IL-10, IL-12, signal transduction, Western blotting
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