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International Immunology, Vol. 14, No. 11, pp. 1247-1253, November 2002
© 2002 Japanese Society for Immunology

Macrophages as main inducers of IFN-{gamma} in T cells following administration of human and mouse heat shock protein 60

Minka Breloer1, Solveig H. Moré1, Anke Osterloh1, Felix Stelter2, Robert S. Jack2 and Arne von Bonin1

1 Bernhard-Nocht-Institute for Tropical Medicine, Bernhard-Nocht Strasse 74, 20359 Hamburg, Germany 2 Institute of Immunology and Transfusion Medicine, Ernst-Moritz-Arndt-University, 17489 Greifswald, Germany

Correspondence to: A. von Bonin; E-mail: Arne_von_Bonin{at}magicvillage.de
Transmitting editor: S. H. E. Kaufman

Human Hsp60 (hHsp60) elicits a potent pro-inflammatory response in cells of the innate immune system. Here we compared the capacity of peritoneal exudate cells (PEC) and bone marrow-derived dendritic cells (DC) to stimulate murine T cells in the presence of Hsp60. Hsp60 induced a specific secretion of high amounts of IFN-{gamma} in T cells with PEC as antigen-presenting cells (APC). Although DC are highly efficient APC, they were much less potent as inducers of IFN-{gamma} in the presence of Hsp60. The IFN-{gamma}-inducing effect of Hsp60 is dependent on co-stimulatory signals provided by B7–CD28 interactions. In addition to hHsp60, we used syngenic murine recombinant Hsp60 (mHsp60) and show that mHsp60 also induces IFN-{gamma} in TCR transgenic T cells. These results demonstrate that mHsp60 as an endogenous ‘self’ molecule can induce an inflammatory response. Interestingly, mHsp60, although sharing >98% protein sequence identity with the hHsp60 homologue, does not bind to human CD14 molecules. Taken together, our results indicate a finely tuned activation of cells from the innate and adaptive immune system by ‘self’ Hsp60 that depends strongly on the type of APC.

Keywords: antigen-presenting cell, danger signal, dendritic cell, Hsp60


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A. Osterloh, U. Kalinke, S. Weiss, B. Fleischer, and M. Breloer
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