Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (5)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Endo, Y.
Right arrow Articles by Fujita, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Endo, Y.
Right arrow Articles by Fujita, T.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

International Immunology, Vol. 14, No. 10, pp. 1193-1201, October 2002
© 2002 Japanese Society for Immunology

Functional characterization of human mannose-binding lectin-associated serine protease (MASP)-1/3 and MASP-2 promoters, and comparison with the C1s promoter

Yuichi Endo1, Minoru Takahashi1, Mikio Kuraya1, Misao Matsushita1, Cordula M. Stover2, Wilhelm J. Schwaeble2 and Teizo Fujita1

1 Department of Biochemistry, Fukushima Medical University School of Medicine, 1-Hikarigaoka, Fukushima 960-1295, Japan 2 Department of Microbiology and Immunology, University of Leicester, Leicester LE1 9HN, UK

Correspondence to: Y. Endo; E-mail: yendo{at}fmu.ac.jp
Transmitting editor: K. Sugamura

The 5'-flanking regions of the genes encoding human mannose-binding lectin-associated serine protease (MASP)-1/3 and MASP-2, key enzymes in the lectin complement pathway, were isolated and characterized. The features of their promoters were compared with those of the human gene for C1s, the effector component of the classical pathway. The sequences upstream from the transcription start sites of the three genes contained the elements essential for transcription and liver-specific expression. Transient expression of constructs of these genes fused to the luciferase reporter gene confirmed their liver-specific expression and showed that the MASP promoters were slightly up-regulated by the presence of IL-1ß. The stimulatory effects of IL-1ß on MASP1/3 and MASP2 gene expression were abolished by the simultaneous presence of IL-6. MASP-1/3 promoter activity was also down-regulated by IFN-{gamma}. In contrast, C1s promoter activity was strongly up-regulated by IL-6, IL-1ß and IFN-{gamma}. These results indicate that IL-6 and IFN-{gamma} affect the expression of the MASP genes in a different fashion from that of the C1s gene, implying differential regulatory effects of these cytokines on the biosynthesis of lectin pathway-specific serine proteases and classical pathway-specific serine proteases.

Keywords: C1s, classical pathway, complement, cytokine, lectin complement pathway, luciferase reporter assay, mannose-binding lectin-associated serine protease, promoter, serine protease


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nephrol Dial TransplantHome page
S. Sagedal, S. Thiel, T. K. Hansen, T. E. Mollnes, H. Rollag, and A. Hartmann
Impact of the complement lectin pathway on cytomegalovirus disease early after kidney transplantation
Nephrol. Dial. Transplant., June 24, 2008; (2008) gfn355v1.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.