International Immunology, Vol. 13, No. 7, 871-876,
July 2001
© 2001 Japanese Society for Immunology
B cell co-receptors regulating T cell-dependent antibody production in common variable immunodeficiency: CD27 pathway defects identify subsets of severely immuno-compromised patients
1 Division of Immunology, Institute for Medical Science, Dokkyo University School of Medicine, Tochigi 321-0293, Japan
2 Department of Infectious Disease and Immunology, Okinawa-Asia Research Center of Medical Science, University of the Ryukyus School of Medicine, Okinawa 903-0215, Japan
3 Division of Tumor Immunology, Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute; Department of Medicine, Harvard Medical School, Boston, MA 02115, USA
Correspondence to: S. Jacquot
CD27 and CD134 ligand (CD134L) are two B cell co-receptors for Th cell activation-induced ligands (i.e. CD70 and CD134) that promote differentiation of B cells into plasma cells and high-rate antibody production respectively. We explored the CD27 pathway and T cell CD134 expression in common variable immunodeficiency (CVID), a disease characterized by a lack of plasma cells and low Ig serum levels. Twelve patients were compared to seven healthy controls. We found a low percentage of circulating CD27+ B cells in seven patients and B cell CD27 expression was not up-regulated by in vitro activation in two of them. Importantly, the number of circulating CD27+ B cells was correlated with the severity of the disease—the patients with the lowest CD27+ B cell counts having the lowest serum Ig concentrations and the lowest total peripheral blood B cell counts. In contrast, CD70 and CD134 were normally expressed on in vitro activated T cells. CD134L was not detected on patient and control B cells in our activation conditions. Functional studies of in vitro Ig production demonstrated an absence of B cell response to CD27 cross-linking, in particular in a patient with normal CD27 expression. Our results indicate that a defect in CD27 expression or function contributes to the pathogenesis of certain severe forms of CVID.
Keywords: memory B cells, T-B interactions, tumor necrosis factor receptor family
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  C. Cunningham-Rundles, L. Radigan, A. K. Knight, L. Zhang, L. Bauer, and A. Nakazawa TLR9 Activation Is Defective in Common Variable Immune Deficiency J. Immunol., February 1, 2006; 176(3): 1978 - 1987. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Iwamoto, M. Ishida, K. Takahashi, K. Takeda, and A. Miyazaki Lipopolysaccharide stimulation converts vigorously washed dendritic cells (DCs) to nonexhausted DCs expressing CD70 and evoking long-lasting type 1 T cell responses J. Leukoc. Biol., August 1, 2005; 78(2): 383 - 392. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Andersen, H. Permin, V. Andersen, L. Schejbel, P. Garred, A. Svejgaard, and T. Barington Deficiency of somatic hypermutation of the antibody light chain is associated with increased frequency of severe respiratory tract infection in common variable immunodeficiency Blood, January 15, 2005; 105(2): 511 - 517. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Garcia, A. B. de Heredia, T. Bellon, E. Carpio, M. Llano, E. Caparros, P. Aparicio, and M. Lopez-Botet Signalling via CD70, a member of the TNF family, regulates T cell functions J. Leukoc. Biol., July 1, 2004; 76(1): 263 - 270. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. F. Fecteau and S. Neron CD40 Stimulation of Human Peripheral B Lymphocytes: Distinct Response from Naive and Memory Cells J. Immunol., November 1, 2003; 171(9): 4621 - 4629. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Warnatz, A. Denz, R. Drager, M. Braun, C. Groth, G. Wolff-Vorbeck, H. Eibel, M. Schlesier, and H. H. Peter Severe deficiency of switched memory B cells (CD27+IgM-IgD-) in subgroups of patients with common variable immunodeficiency: a new approach to classify a heterogeneous disease Blood, March 1, 2002; 99(5): 1544 - 1551. [Abstract] [Full Text] [PDF]
|
|