Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (22)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Kawamoto, S.
Right arrow Articles by Miyazaki, J.-i.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kawamoto, S.
Right arrow Articles by Miyazaki, J.-i.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

International Immunology, Vol. 13, No. 5, 685-694, May 2001
© 2001 Japanese Society for Immunology

Suppression of Th1 cell activation and prevention of autoimmune diabetes in NOD mice by local expression of viral IL-10

Shunsuke Kawamoto1,2, Yoshio Nitta1,2, Fumi Tashiro1, Atsushi Nakano1, Eiji Yamato1, Hideaki Tahara3, Koichi Tabayashi2 and Jun-ichi Miyazaki1

Department of Nutrition and Physiological Chemistry, Osaka University Medical School, Suita, Osaka 565-0871, Japan
Department of Thoracic and Cardiovascular Surgery, Tohoku University School of Medicine, Sendai, Miyagi 980-8574, Japan
Department of Surgery, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan

Correspondence to: J.-i. Miyazaki

Insulin-dependent diabetes mellitus in the NOD mouse model is caused by the T cell-mediated autoimmune destruction of pancreatic ß cells. Viral IL-10 (vIL-10), encoded in the Epstein–Barr virus genome, shares many of the anti-inflammatory properties of cellular IL-10, but lacks its immunostimulatory properties. In the present study, we generated transgenic (Tg) NOD mice in which vIL-10 was produced exclusively in pancreatic islets and investigated the effect of vIL-10 on the development of diabetes. The accumulation of lymphocytes around islets was more prominent, but the invasive insulitis decreased in the vIL-10 Tg mice. The incidence of diabetes was markedly reduced in the vIL-10 Tg mice, in clear contrast to the accelerated diabetes seen in the murine IL-10 Tg NOD mice. IL-12p40 and IFN-{gamma} mRNA levels were decreased in pancreata of the vIL-10 Tg mice, although CD4 mRNA level was markedly increased. These results suggest that locally produced vIL-10 induced leukocyte migration, but inhibited the activation of Th1, probably through suppressing the production of IL-12. They indicate that vIL-10 may well be superior to cellular IL-10 in the treatment of autoimmune diabetes. The vIL-10 Tg NOD mice should provide a useful tool for understanding the differential action of vIL-10 versus cellular IL-10.

Keywords: autoimmunity, cytokines, diabetes, NOD mouse, transgenic mouse

Transmitting editor: S.-i. Nishikawa


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Sci SignalHome page
K. Yamazaki, J. Gohda, A. Kanayama, Y. Miyamoto, H. Sakurai, M. Yamamoto, S. Akira, H. Hayashi, B. Su, and J.-i. Inoue
Two Mechanistically and Temporally Distinct NF-{kappa}B Activation Pathways in IL-1 Signaling
Sci. Signal., October 20, 2009; 2(93): ra66 - ra66.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
K. A. Smith, S. Efstathiou, and A. Cooke
Murine Gammaherpesvirus-68 Infection Alters Self-Antigen Presentation and Type 1 Diabetes Onset in NOD Mice
J. Immunol., December 1, 2007; 179(11): 7325 - 7333.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. Lucas and G. McFadden
Secreted Immunomodulatory Viral Proteins as Novel Biotherapeutics
J. Immunol., October 15, 2004; 173(8): 4765 - 4774.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
Z. Yang, M. Chen, R. Wu, L. B. Fialkow, J. S. Bromberg, M. McDuffie, A. Naji, and J. L. Nadler
Suppression of Autoimmune Diabetes by Viral IL-10 Gene Transfer
J. Immunol., June 15, 2002; 168(12): 6479 - 6485.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
J. M. Phillips, N. M. Parish, M. Drage, and A. Cooke
Cutting Edge: Interactions Through the IL-10 Receptor Regulate Autoimmune Diabetes
J. Immunol., December 1, 2001; 167(11): 6087 - 6091.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.