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International Immunology, Vol. 13, No. 5, 665-674, May 2001
© 2001 Japanese Society for Immunology

The 3' Ig{kappa} enhancer contains RNA polymerase II promoters: implications for endogenous and transgenic {kappa} gene expression

Hong Ming Shen, Andrew Peters,, Daniel Kao and Ursula Storb3

1 Biochemistry and Molecular Biology, University of Chicago, 920 East 58th Street, Chicago, IL 60637, USA
Departments of Molecular Genetics and Cell Biology, and

Correspondence to: U. Storb

We have created a {kappa} transgene in which a polymerase (pol) III promoter replaces the pol II promoter. Two independent transgenic lines show somatic hypermutation of the transgene in B cells from hyperimmunized mice. Both lines transcribe transgenes from the pol III promoter in the liver. However, in spleen and spleen B cell-derived hybridomas, they also transcribe mRNA from pol II promoters located within the 3' {kappa} enhancer of the preceding transgene copy in a tandem transgene array. The findings demonstrate that in an array of multiple transgenes the expression (and somatic hypermutation) of an individual transgene copy must be considered in the context of the other copies. We also show that sequences around the 3' {kappa} enhancer in endogenous genes are transcribed. The possible role of these promoters in endogenous {kappa} gene expression is discussed. An unrelated finding in this study was a novel RNA splice in one hybridoma.

Keywords: Ig genes, non-consensus RNA splicing, RNA polymerase II, RNA polymerase III, somatic hypermutation

Transmitting editor: K. Knight


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