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International Immunology, Vol. 13, No. 5, 607-613, May 2001
© 2001 Japanese Society for Immunology

Antigen-specific Th1 cells as direct effectors of Propionibacterium acnes-primed lipopolysaccharide-induced hepatic injury

Takahiro Okazaki,2, Shoichi Ozaki,3, Tetsuya Nagaoka1,, Masako Kozuki, Satoshi Sumita, Masao Tanaka, Fumio Osakada1,, Masaaki Kishimura1,, Tetsu Kakutani1, and Kazuwa Nakao

1 Takasago Research Laboratories, Kaneka Corp., Takasago, Hyogo 676-8688, Japan
Department of Medicine and Clinical Science, Kyoto University Graduate School of Medicine, 54 Shogoin-kawahara-cho, Sakyo-ku, Kyoto 606-8507, Japan

Correspondence to: S. Ozaki, Department of Rheumatic and Allergic Diseases, St Marianna University School of Medicine, Sugao, Miyamae-ku, Kawasaki 216-8512, Japan

Th1 cells are cytotoxic effector cells that utilize Fas ligand (FasL) and tumor necrosis factor. The physiological roles of cytotoxic Th1 cells are considered to be immunoregulation by eliminating autoreactive lymphocytes or hyper-activated foreign antigen-specific lymphocytes. Their pathological roles, however, remain to be clarified. To investigate whether Th1 cells can destroy organs, we generated a Propionibacterium acnes-specific Th1 clone from C57BL/6 mice and tested whether the clone could serve as an effector in a P. acnes-primed lipopolysaccharide (LPS)-induced hepatic injury system, one of the septic shock models. B6Smn.C3H-FasLgld (B6-gld) mice, which were deficient in functional FasL, were resistant to P. acnes/LPS-induced hepatic shock. The Th1 clone rendered B6-gld mice sensitive to the hepatic shock after the i.v. transfer. The hepatic injury in the clone-transferred B6-gld mice, which was evaluated by both biochemical and histological examination, was inhibited by an anti-FasL mAb that we developed. These results suggested that bacterial antigen-specific Th1 cells like this clone can participate in organ destruction in vivo as one of the cytotoxic effectors and play a critical role in endotoxin-induced hepatic injury.

Keywords: cytotoxicity, endotoxin shock, lipopolysaccharide rodent, Th1/Th2

2 Present address: Molecular Immunogenetics and Vaccine Research Section, Metabolism Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892-1578, USA

3 Present address: Department of Rheumatic and Allergic Diseases, St Marianna University School of Medicine, Sugao, Miyamae-ku, Kawasaki 216-8512, Japan

Transmitting editor: S. Nagata


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