International Immunology, Vol. 13, No. 4, 421-429,
April 2001
© 2001 Japanese Society for Immunology
Chondrocyte antigen expression, immune response and susceptibility to arthritis
1 The Edward Jenner Institute for Vaccine Research, Compton, Newbury, Berkshire RG20 7NN, UK
2 Department Biochemistry, The University of Hong Kong, Sassoon Road, Hong Kong, China
Correspondence to: H. C. Bodmer
The association of HLA-B27 with certain forms of arthritis implies a role for MHC class I-restricted T cells in the arthritic process. Our aim was to study CD8+ T cell responses towards specific antigens localized in joint tissue. Known determinants were introduced into chondrocytes of transgenic (TG) mice, under the control of the cis-regulatory sequences of the human type II collagen gene (COL2A1). Two Escherichia coli ß-galactosidase (ß-gal)-expressing lines were derived (CIIL73 and CIIL64) as well as two lines (CIINP) expressing influenza A virus nucleoprotein (NP). Expression of the antigens could be demonstrated in cartilaginous tissues. The TG lines showed variable degrees of responsiveness towards the transgene-introduced antigens; whilst 75% of CIIL73 mice had an impaired cytotoxic T lymphocyte (CTL) response towards ß-gal, the response in CIIL64 mice was essentially normal. However, both lines displayed normal proliferative and antibody responses to ß-gal. A reduced CTL response was seen to NP in the CIINP lines in ~65% of the animals. In spite of the persistence of T cell responses to the transgene antigens in these lines, induction of CTL responses alone has so far failed to induce clinical signs of arthritis. Interestingly, some animals expressing ß-gal were susceptible to arthritis following challenge with type II collagen alone, whilst their non-TG littermates and TG mice from other lines remained unaffected. As ß-gal is expressed by E. coli, a component of the normal gut flora, this suggests a possible role for gut-derived immune responses. We believe these lines could form the basis of a model for studying links between intestinal inflammation and arthritis.
Keywords: ß-galactosidase, ankylosing spondylitis, autoimmunity, BALB/c, collagen, cytotoxic T lymphocyte, influenza A virus, mice, nucleoprotein, rodent, tolerance
3 Present address: Department of Biophysics, University of Toronto, Ontario Cancer Institute, 610, University Avenue, Toronto, Ontario M5G 2M9, Canada
Transmitting editor: E. Simpson
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