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International Immunology, Vol. 13, No. 3, 367-376, March 2001
© 2001 Japanese Society for Immunology

Comprehensive gene expression profile of human activated Th1- and Th2-polarized cells

Shigenori Nagai, Shin-ichi Hashimoto, Taro Yamashita, Nobuaki Toyoda, Taku Satoh, Takuji Suzuki and Kouji Matsushima

Department of Molecular Preventive Medicine, School of Medicine and CREST, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan

Correspondence to: K. Matsushima

In response to antigen stimulation, Th cells differentiate into two types of effector cells, Th1 and Th2. Th1 cells predominantly mediate cellular immunity, whereas Th2 cells induce humoral allergic responses. We have conducted here serial analysis of gene expression (SAGE) in human activated Th1- and Th2-polarized cells from cord blood. SAGE analysis of 64,510 tags (32,219 and 32,291 tags from Th1 and Th2 cells respectively) allowed identification of 22,096 different transcripts. In activated Th1 cells, many of the known genes (12 genes, P < 0.01; 56 genes, P < 0.05), including genes encoding IFN-{gamma}, lymphotactin, osteopontin, MIP-1{alpha}, MIP-1ß, perforin, ß-catenin and CD55, are highly expressed. On the other hand, in activated Th2 cells rather limited numbers of known genes (four genes, P < 0.01; 10 genes; P < 0.05), such as genes encoding FUS, ILF-2, IL-13 and E2-EPF, are found to be selectively expressed. The comprehensive identification of genes selectively expressed in human activated Th1 or Th2 cells should contribute to our understanding of the molecular basis of Th1/Th2-dominated human diseases and may provide genetic information to diagnose these diseases.

Keywords: chemokine, cytokine, serial analysis of gene expression, Th, transcripts

Transmitting editor: M. Miyasaka


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