International Immunology, Vol. 13, No. 2, 127-134,
February 2001
© 2001 Japanese Society for Immunology
Association of distinct tetraspanins with MHC class II molecules at different subcellular locations in human immature dendritic cells
1 Basel Institute for Immunology, Grenzacherstrasse 487, CH-4005 Basel, Switzerland
2 Department of Cell Biology and Immunology, Vrije Universiteit, 1007 MB Amsterdam, The Netherlands
Correspondence to: Correspondence to:J. Pieters
Dendritic cells have the capacity to trigger T cell responses in lymphoid organs against antigens captured in the periphery. T cell stimulation depends on the ability of MHC class II molecules to present peptides at the cell surface that are acquired in MHC class II compartments. The high capacity of dendritic cells to stimulate T lymphocytes is related to their ability to regulate the distribution of MHC class II molecules intracellularly. To analyze the molecular components involved in the generation of MHC class II–peptide complexes in human immature dendritic cells, mAb were raised against purified MHC class II compartments. One of the antigens turned out to be CD63, a member of the tetraspanin superfamily. CD63 localized exclusively intracellularly where it associated with peptide-loaded class II molecules. In contrast, the tetraspanins CD9, CD53 and CD81 associated with class II molecules at the plasma membrane. Selective association of distinct tetraspanins may be involved in the regulation of MHC class II distribution in human dendritic cells.
Keywords: antigen presentation, antigen processing, dendritic cells, MHC class II, MHC class II compartments, tetraspanins
Transmitting editor: L. Du Pasquier
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