International Immunology, Vol. 13, No. 12, 1583-1593,
December 2001
© 2001 Japanese Society for Immunology
Role of B cells as antigen-presenting cells in vivo revisited: antigen-specific B cells are essential for T cell expansion in lymph nodes and for systemic T cell responses to low antigen concentrations
Department of Molecular Genetics and Microbiology, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, NJ 08854, USA
Correspondence to: Y. Ron
Studies in B cell-deficient mice generated by continuous injection of anti-µ antibodies (µSM) showed that T cell priming in lymph nodes was dependent on antigen presentation by B cells. This concept has recently become controversial since a wide range, from complete deficiency to near normal T cell responses, was reported in studies carried out with B cell-deficient mice generated by gene disruption (µMT). In this study we show that in the absence of B cells, T cell responses are greatly reduced in all the available µMT mouse strains although responses in µMT of the C57BL/6 background (which were used for most studies with µMT) were much more variable and could reach up to 42% of control. In contrast, T cell responses in µMT 
F1 bone marrow chimeras which have the same phenotype as µMT were totally impaired, suggesting a principle difference between mice developing without B cells (µMT mice) and µSM which are made B cell deficient only after birth. Normal T cell priming was completely restored by reconstitution of µMT and µMT F1 mice with syngeneic B cells. Interestingly, only B cell populations containing antigen-specific B cells were capable of reconstituting T cell responses. Monoclonal B cells taken from Ig transgenic mice could not reconstitute responses to an irrelevant antigen. We also found that B cells were also required for systemic T cell priming when antigen concentrations were limiting but were not required for priming (for T cell help) when mice were immunized with a high antigen dose.
Keywords: antigen presentation, B cell-deficient mice, clonal expansion, T-B cell collaboration, T cell activation
Transmitting editor: D. R. Green
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