Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (9)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Murakami, S.
Right arrow Articles by Okada, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Murakami, S.
Right arrow Articles by Okada, H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

International Immunology, Vol. 13, No. 12, 1533-1540, December 2001
© 2001 Japanese Society for Immunology

Adenosine regulates the IL-1ß-induced cellular functions of human gingival fibroblasts

Shinya Murakami, Tomoko Hashikawa, Teruyuki Saho, Masahide Takedachi, Takenori Nozaki, Yoshio Shimabukuro and Hiroshi Okada

Department of Periodontology, Division of Oral Biology and Disease Control, Osaka University Graduate School of Dentistry, 1-8 Yamadaoka, Suita, Osaka 565-0871, Japan

Correspondence to: S. Murakami

In this study we examined the influence of adenosine on the cellular functions of human gingival fibroblasts (HGF), such as the production of inflammatory cytokines and extracellular matrices (ECM), and the expression and function of adhesion molecules. Concerning the expression of adenosine receptors, RT-PCR analysis revealed that HGF expressed adenosine receptor A1, A2a and A2b, but not A3 mRNA. Ligation of adenosine receptors by adenosine or its related analogue, 2-chloroadenosine (2-CADO), N6-cyclopentyladenosine (CPA) or CGS21680 synergistically increased IL-1ß-induced IL-6 and IL-8 production. In terms of ECM expression, adenosine and the adenosine receptor agonists, 2-CADO and CPA, enhanced constitutive and IL-1ß-induced expression of hyaluronate synthase mRNA, but not the mRNA levels of other ECM, such as collagen type I, III and fibronectin. Moreover, the adherence of IL-1ß-stimulated HGF to activated lymphocytes was also inhibited by adenosine, which is in part explained by the fact that adenosine down-regulated the IL-1ß-induced expression of ICAM-1 on HGF. These results provide new evidence for the possible involvement of adenosine in the regulation of inflammatory responses in periodontal tissues.

Keywords: adenosine, cytokines, fibroblasts, human, immunomodulators, inflammation

Transmitting editor: T. Hamaoka


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Dent. Res.Home page
T. Hashikawa, M. Takedachi, M. Terakura, S. Yamada, L.F. Thompson, Y. Shimabukuro, and S. Murakami
Activation of adenosine receptor on gingival fibroblasts.
J. Dent. Res., August 1, 2006; 85(8): 739 - 744.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
A. Fortin, D. Harbour, M. Fernandes, P. Borgeat, and S. Bourgoin
Differential expression of adenosine receptors in human neutrophils: up-regulation by specific Th1 cytokines and lipopolysaccharide
J. Leukoc. Biol., March 1, 2006; 79(3): 574 - 585.
[Abstract] [Full Text] [PDF]

Home page
 J. Dent. Res.Home page
T. Hashikawa, M. Takedachi, M. Terakura, T. Saho, S. Yamada, L.F. Thompson, Y. Shimabukuro, and S. Murakami
Involvement of CD73 (ecto-5'-nucleotidase) in Adenosine Generation by Human Gingival Fibroblasts
J. Dent. Res., November 1, 2003; 82(11): 888 - 892.
[Abstract] [Full Text] [PDF]

Home page
 J. Dent. Res.Home page
S. Murakami, N. Yoshimura, H. Koide, J. Watanabe, M. Takedachi, M. Terakura, M. Yanagita, T. Hashikawa, T. Saho, Y. Shimabukuro, et al.
Activation of Adenosine-receptor-enhanced iNOS mRNA Expression by Gingival Epithelial Cells
J. Dent. Res., April 1, 2002; 81(4): 236 - 240.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.