Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (8)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Flesch, I. E. A.
Right arrow Articles by Reimann, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Flesch, I. E. A.
Right arrow Articles by Reimann, J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

International Immunology, Vol. 12, No. 9, 1365-1370, September 2000
© 2000 Japanese Society for Immunology

Monocyte inflammatory protein-1{alpha} facilitates priming of CD8+ T cell responses to exogenous viral antigen

Inge E. A. Flesch, Detlef Stober, Reinhold Schirmbeck and Jörg Reimann

Department of Medical Microbiology and Immunology, University of Ulm, Helmholtzstrasse 8/1, 89081 Ulm, Germany

Correspondence to: J. Reimann

Dendritic cells (DC) derived from bone marrow precursors of BALB/c or C57BL/6 mice in low-serum cultures supplemented with granulocyte macrophage colony stimulating factor and Flt3 ligand were pulsed in vitro with hepatitis B surface antigen (HBsAg) particles. DC processed exogenous HBsAg and presented its MHC class I-binding epitopes to cytotoxic T lymphocytes (CTL). This specific and restricted interaction of DC with CTL stimulated release of IFN-{gamma} and macrophage inflammatory protein (MIP)-1{alpha} from the responding CTL. MIP-1{alpha} enhanced the survival of DC in vitro but did not induce proliferation. Furthermore, co-delivery of MIP-1{alpha} facilitated CTL priming in vivo to exogenous HBsAg in low responder C57BL/6 (H-2b) mice: a single injection of a low dose of HBsAg particles (without further adjuvants) successfully primed Kb-restricted CTL responses to HBsAg only when the exogenous antigen was co-delivered with 100 ng MIP-1{alpha}. These in vitro and in vivo data point to an important role of MIP-1{alpha} in the DC-dependent priming of CTL to exogenous viral antigens.

Keywords: chemokines, cytotoxic T lymphocyte priming, dendritic cells

Transmitting editor: T. Hünig


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Immunol.Home page
O. Bruna-Romero, J. Schmieg, M. Del Val, M. Buschle, and M. Tsuji
The Dendritic Cell-Specific Chemokine, Dendritic Cell-Derived CC Chemokine 1, Enhances Protective Cell-Mediated Immunity to Murine Malaria
J. Immunol., March 15, 2003; 170(6): 3195 - 3203.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
H. W. van Deventer, J. S. Serody, K. P. McKinnon, C. Clements, W. J. Brickey, and J. P.-Y. Ting
Transfection of Macrophage Inflammatory Protein 1{alpha} into B16 F10 Melanoma Cells Inhibits Growth of Pulmonary Metastases But Not Subcutaneous Tumors
J. Immunol., August 1, 2002; 169(3): 1634 - 1639.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.