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International Immunology, Vol. 12, No. 9, 1365-1370, September 2000
© 2000 Japanese Society for Immunology

Monocyte inflammatory protein-1{alpha} facilitates priming of CD8+ T cell responses to exogenous viral antigen

Inge E. A. Flesch, Detlef Stober, Reinhold Schirmbeck and Jörg Reimann

Department of Medical Microbiology and Immunology, University of Ulm, Helmholtzstrasse 8/1, 89081 Ulm, Germany

Correspondence to: J. Reimann

Dendritic cells (DC) derived from bone marrow precursors of BALB/c or C57BL/6 mice in low-serum cultures supplemented with granulocyte macrophage colony stimulating factor and Flt3 ligand were pulsed in vitro with hepatitis B surface antigen (HBsAg) particles. DC processed exogenous HBsAg and presented its MHC class I-binding epitopes to cytotoxic T lymphocytes (CTL). This specific and restricted interaction of DC with CTL stimulated release of IFN-{gamma} and macrophage inflammatory protein (MIP)-1{alpha} from the responding CTL. MIP-1{alpha} enhanced the survival of DC in vitro but did not induce proliferation. Furthermore, co-delivery of MIP-1{alpha} facilitated CTL priming in vivo to exogenous HBsAg in low responder C57BL/6 (H-2b) mice: a single injection of a low dose of HBsAg particles (without further adjuvants) successfully primed Kb-restricted CTL responses to HBsAg only when the exogenous antigen was co-delivered with 100 ng MIP-1{alpha}. These in vitro and in vivo data point to an important role of MIP-1{alpha} in the DC-dependent priming of CTL to exogenous viral antigens.

Keywords: chemokines, cytotoxic T lymphocyte priming, dendritic cells

Transmitting editor: T. Hünig


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