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International Immunology, Vol. 12, No. 9, 1303-1309, September 2000
© 2000 Japanese Society for Immunology

Presence of the 55 kDa glycosylation inhibiting factor in human serum

Kaoru Kasahara1, Tatsumi Nakano1,4, Hiroki Takahashi1, Yasuyuki Ishii2, Kimishige Ishizaka3 and Kohzoh Imai1

1 First Department of Internal Medicine, Sapporo Medical University, South 1 West 17, Sapporo 060-8543, Japan
2 Kirin Pharmaceutical Laboratory, Takasaki 370-1295, Japan
3 La Jolla Institute for Allergy and Immunology, San Diego, CA 92121, USA

Correspondence to: T. Nakano, Jikeikai Hospital, 6-50 Asahigaoka 5, Chuo-ku, Sapporo 064-8575, Japan

An ELISA system for the human glycosylation inhibiting factor (GIF) was established using polyclonal antibodies against highly purified 13 kDa recombinant human GIF, and the concentration of GIF in the sera of healthy donors and patients with various diseases was determined. GIF was detected in the sera of most healthy individuals and its concentration tended to increase with age. It was also found that the serum GIF levels markedly increased in some patients with rheumatoid arthritis or malignant tumors. Analysis of serum samples by SDS–PAGE and immunoblotting revealed a 55 kDa protein that has both the GIF antigenic determinant and the TCR {alpha} chain determinant. A 13 kDa GIF was not detected in the sera. In view of our previous findings on antigen-specific GIF from murine suppressor T cell hybridomas indicating that the 55 kDa GIF is a post-translationaly formed conjugate of a TCR {alpha} chain with 13 kDa GIF, we suspect that the 55 kDa GIF detected in human sera is a human homologue of the murine 55 kDa GIF.

Keywords: aging, malignant tumor, rheumatoid arthritis, Ts cell

4 Present address: Jikeikai Hospital, 6-50 Asahigaoka 5, Chuo-ku, Sapporo 064-8575, Japan

Transmitting editor: M. Taniguchi


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