International Immunology, Vol. 12, No. 7, 1095-1103,
July 2000
© 2000 Japanese Society for Immunology
Peripheral blood extrathymic CD4+CD8+ T cells with high cytotoxic activity are from the same lineage as CD4+CD8 T cells in cynomolgus monkeys
1 Tsukuba Primate Center, National Institute of Infectious Diseases, 1 Hachimandai, Tsukuba, Ibaraki 305-0843, Japan
2 Department of Virology, School of Medicine, University of Tokushima, 3 Kuramoto, Tokushima 770-8503, Japan
3 Department of Biomedical Science, Faculty of Agriculture and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
Correspondence to: K. Terao
We have previously reported that CD4/CD8 double-positive (DP) T cells with the resting memory phenotype are present in the periphery of healthy cynomolgus monkeys. In the present study, we performed functional studies on the T cells. The expression of CD4 and CD8 on DP, CD4 single-positive (SP) or CD8 SP T cells was stable in cultures with either mitogen or anti-CD3 antibody stimulation. In spite of lacking CD28 expression, DP T cells showed similar proliferative ability and apoptosis sensitivity to CD4 SP and CD8 SP T cells. DP T cells showed both helper and cytotoxic activities. Although the helper activity of DP T cells was lower than that of CD4 SP T cells, cytotoxic activity was comparable to that of CD8 SP T cells. Fresh DP T cells killed target cells mainly by the perforingranzyme pathway. In addition, fresh DP T cells expressed a high level of mRNA for IFN-
and produced a high level of IFN-
when they were activated by anti-CD3 antibody ligation. On the other hand, several expanded DP T cell clones shared TCR Vß with expanded CD4 SP T cell clones, strongly suggesting that those two corresponding clones with DP and CD4 SP phenotypes might be derived from the same ancestor T cell. These results showed that the DP T cells are a novel T cell subset with functions overlapping with those of CD4 SP and CD8 SP T cells, and that they might play protective and regulatory roles in secondary immune response in cynomolgus monkeys.
Keywords: function, non-human primate, origin
Transmitting editor: K. Okumura
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