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International Immunology, Vol. 12, No. 5, 731-735, May 2000
© 2000 Japanese Society for Immunology

DEC-205 as a marker of dendritic cells with regulatory effects on CD8 T cell responses

Vadim Kronin, Li Wu, Schiaoching Gong1, Michel C Nussenzweig1 and Ken Shortman

The Walter and Eliza Hall Institute of Medical Research, Post Office Royal Melbourne Hospital, Melbourne, Victoria 3050, Australia
1 The Rockefeller University, Howard Hughes Medical Institute, New York, NY 10021, USA

Correspondence to: K. Shortman

We have previously reported that a population of lymphoid-related CD8{alpha}+ DEC-205+ dendritic cells (DC) from mouse spleen have `regulatory' effects on the T cells they activate. CD8 T cells produce IL-2 and give a sustained proliferative response to allogeneic CD8{alpha}– DEC-205– splenic DC, but produce little IL-2 and give a limited response to allogeneic CD8+ DEC-205+ splenic DC. Although CD8{alpha} and DEC-205 correlate closely among splenic DC, lymph nodes (LN) include a large population of CD8{alpha}low DEC-205high DC. By i.v. transfer of purified thymic early lymphoid precursors into irradiated recipient mice we now demonstrate that these CD8{alpha}low but DEC-205high LN DC can be the progeny of a lymphoid precursor population, apparently corresponding to the CD8{alpha}high DEC-205high DC progeny of the same precursors in spleen and thymus. By culture of the separated, purified DC with allogeneic CD8 T cells we demonstrate that the CD8{alpha}low DEC-205high DC of LN are also functionally equivalent to the CD8{alpha}high DEC-205high DC of spleen. Therefore, DEC-205 but not CD8{alpha} serves to segregate functionally distinct DC types in LN. However, DC isolated from the spleens of genetically manipulated DEC-205null mice and separated on the basis of CD8{alpha} expression have a similar capacity to stimulate CD8 T cells as their heterozygous littermate controls, with the CD8{alpha}+ but now DEC-205null DC still giving restricted responses. In conclusion, high expression of DEC-205 appears to be a good marker of the lymphoid-related regulatory type of DC, but DEC-205 itself is not responsible for transmitting negative signals to the T cells.

Keywords: CD8, DEC-205, IL-2 production, regulatory dendritic cells

Transmitting editor: M. Feldmann


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