International Immunology, Vol. 12, No. 5, 711-719,
May 2000
© 2000 Japanese Society for Immunology
Short-lived immunization site inflammation in self-limited active experimental allergic encephalomyelitis
1 Fondazione Andrea Cesalpino, Istituto I Clinica Medica, Università `La Sapienza', Rome 00161, Italy
2 Istituto Pasteur-Cenci Bolognetti, Rome 00185, Italy
3 Laboratorio di Fisiopatologia di Organo e di Sistema and
4 Servizio Qualità e Sicurezza Sperimentazione Animale, Istituto Superiore di Sanità, Rome 00161, Italy
Correspondence to: V. Barnaba, Fondazione Andrea Cesalpino, Istituto I Clinica Medica, Università di Roma `La Sapienza', Rome 00161, Italy
To understand the mechanisms underlying spontaneous remission of proteolipid protein (PLP) 139–151 peptide-induced experimental allergic encephalomyelitis (EAE), an acute autoimmune disease of SJL mice resembling human multiple sclerosis, we examined both the effector response site in the central nervous system (CNS) and the immunization site at different phases of the disease. In the CNS, the frequency of PLP 139–151 peptide-specific IFN-
-producing T cells as well as the amount of infiltrating CD4+ and CD11b+ cells decreased with recovery. However, IL-4-producing cells were always rare and cyclooxygenase-2+ cells were numerous only at disease peak in the CNS, suggesting that Th2 cytokines and prostaglandins did not determine remission of EAE. By looking at the s.c. site of PLP 139–151 peptide plus adjuvant injection, we found that, although the inflammatory infiltrate was abundant, CD11b+ cells started to decrease already during disease acute phase and DEC-205+ cells were numerous only at early time points. We propose that immunization site inflammation is short-lived in PLP 139–151 peptide-induced EAE, and this leads to a temporary autoreactive T cell stimulation and to a self-limited disease.
Keywords: adjuvant, autoimmunity, immunization, inflammation, T cell cytokines
5 Present address: Laboratorio di Fisiopatologia, Centro Ricerca Sperimentale, Istituto Regina Elena, via delle Messi d'Oro 156, Rome 00158, Italy
6 Present address: Istituto Nazionale per le Malattie Infettive IRCSS `La Sapienza' CRAIDS, via Portiense 292, Rome 00149, Italy
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