International Immunology, Vol. 12, No. 5, 613-621,
May 2000
© 2000 Japanese Society for Immunology
In vivo identification of lymphocyte subsets exhibiting transcriptionally active NF-
B/Rel complexes
Unité de Biologie Moléculaire de l'Expression Génique, URA 1773 CNRS, Institut Pasteur, 28 rue du Dr Roux, 75724 Paris Cedex 15, France
1 Département d'Hématologie, Hôpital Avicenne, 93000 Bobigny, France
2 Present address: Max-Delbrück-Centrum für Molekulare Medizin, Robert-Rössle-Strasse 10, 13122 Berlin-Buch, Germany
Correspondence to: S. Mémet. Email: symemet{at}pasteur.fr
To analyze the NF-
B/Rel activity pattern in a living organism, we previously generated transgenic mice carrying a B-dependent lacZ gene. In situ analysis of both primary and secondary lymphoid organs revealed a strong NF-B transcriptional activity in antigen-presenting cells, some endothelial cells and sinus lining cells of the lymph node capsula with very little activity in lymphocytes and thymocytes. Using fluorescein-di-ß-D-galactopyranoside (FDG) as a vital substrate for the ß-galactosidase, we re-examined by flow cytometry the NF-B/Rel transcriptional activity in our mouse model. We report here that such constitutive NF-B/Rel activity was significantly detected in thymocytes at the CD44+CD25– stage. This constitutive activity extended with CD25 expression to the majority of the CD44–CD25+ thymocytes and was then restricted to a few mature T cells. In the spleen, constitutive NF-B/Rel activity was found in most B cells, unlike T cells which were largely negative. Virgin IgD+ B cells expressed higher levels of NF-B transcriptional activity than other B cell types. Altogether, these results suggest that NF-B/Rel complexes are key players in the in vivo differentiation of IgD+ B lymphocytes and possibly CD25+ thymocytes.
Keywords: lymphocyte, NF-B, Rel, spleen, thymus, transgenic mice
The first two authors contributed equally to this work
Transmitting editor: A. Fischer
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