Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (30)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Echchakir, H.
Right arrow Articles by Mami-Chouaib, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Echchakir, H.
Right arrow Articles by Mami-Chouaib, F.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

International Immunology, Vol. 12, No. 4, 537-546, April 2000
© 2000 Japanese Society for Immunology

Evidence for in situ expansion of diverse antitumor-specific cytotoxic T lymphocyte clones in a human large cell carcinoma of the lung

Hamid Echchakir, Isabelle Vergnon, Guillaume Dorothée, Dominique Grunenwald1, Salem Chouaib and Fathia Mami-Chouaib

Laboratoire Cytokines et Immunologie des tumeurs Humaines, U487 INSERM, Institut Gustave Roussy, 94805 Villejuif Cedex, France
1 Département Thoracique, Institut Montsouris, 75013 Paris, France

Correspondence to: F. Mami-Chouaib

We have isolated several cytotoxic T lymphocyte (CTL) clones from lymphocytes infiltrating a human large cell carcinoma (LCC) of the lung. All these clones were found to express a CD3+, TCR{alpha}ß+, CD8+, CD4–, CD28– phenotype. According to their TCR ß chain variable region expression, they were divided in three major groups. The first group, including the majority of the clones, expressed a unique Vß3–Jß1.2 TCR. The second group expressed a Vß22–Jß1.4 rearrangement and the third group, including only two clones, expressed a Vß8–Jß1.5 TCR. Functional studies showed that all the CTL clones mediated a high cytotoxic activity against the autologous tumor cell line. While the Vß3+ clones showed a weak lysis against few allogeneic non-small cell lung cancer (NSCLC) tumor cell lines, Vß8+ and Vß22+ T cell clones were able to kill a panel of allogeneic NSCLC tumor cell lines. Cytotoxicity-blocking experiments using specific mAb indicated that, while the Vß3+ and Vß22+ CTL clones were HLA-A2 restricted, the Vß8+ clones appeared HLA-B or -C restricted. TCR transcripts expressed in the cloned cells were determined by CDR3 size and sequence analyses, and compared to those present in fresh tumor tissue. Interestingly, our studies demonstrated that the CTL clones identified in vitro were selectively expanded in vivo at the tumor site as compared to autologous peripheral blood lymphocytes. These results further provide evidence that an immune response may take place in NSCLC and that effector T cells may contribute to tumor regression.

Keywords: cytotoxic T lymphocyte, non-small cell lung cancer, TCR, tumor-associated antigen, tumor-infiltrating lymphocyte

Transmitting editor: J. F. Bach


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Cancer Res.Home page
K. Franciszkiewicz, A. Le Floc'h, A. Jalil, F. Vigant, T. Robert, I. Vergnon, A. Mackiewicz, K. Benihoud, P. Validire, S. Chouaib, et al.
Intratumoral Induction of CD103 Triggers Tumor-Specific CTL Function and CCR5-Dependent T-Cell Retention
Cancer Res., August 1, 2009; 69(15): 6249 - 6255.
[Abstract] [Full Text] [PDF]

Home page
 Vet PatholHome page
A. K. Bauer and E. A. Rondini
REVIEW PAPER: The Role of Inflammation in Mouse Pulmonary Neoplasia
Vet. Pathol., May 1, 2009; 46(3): 369 - 390.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. Z. Noman, S. Buart, J. Van Pelt, C. Richon, M. Hasmim, N. Leleu, W. M. Suchorska, A. Jalil, Y. Lecluse, F. El Hage, et al.
The Cooperative Induction of Hypoxia-Inducible Factor-1{alpha} and STAT3 during Hypoxia Induced an Impairment of Tumor Susceptibility to CTL-Mediated Cell Lysis
J. Immunol., March 15, 2009; 182(6): 3510 - 3521.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
F. El Hage, V. Stroobant, I. Vergnon, J.-F. Baurain, H. Echchakir, V. Lazar, S. Chouaib, P. G. Coulie, and F. Mami-Chouaib
Preprocalcitonin signal peptide generates a cytotoxic T lymphocyte-defined tumor epitope processed by a proteasome-independent pathway
PNAS, July 22, 2008; 105(29): 10119 - 10124.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
A. L. Floc'h, A. Jalil, I. Vergnon, B. L. M. Chansac, V. Lazar, G. Bismuth, S. Chouaib, and F. Mami-Chouaib
{alpha}E{beta}7 integrin interaction with E-cadherin promotes antitumor CTL activity by triggering lytic granule polarization and exocytosis
J. Exp. Med., March 19, 2007; 204(3): 559 - 570.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. Abouzahr, G. Bismuth, C. Gaudin, O. Caroll, P. Van Endert, A. Jalil, J. Dausset, I. Vergnon, C. Richon, A. Kauffmann, et al.
Identification of target actin content and polymerization status as a mechanism of tumor resistance after cytolytic T lymphocyte pressure
PNAS, January 31, 2006; 103(5): 1428 - 1433.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
B. Le Maux Chansac, A. Moretta, I. Vergnon, P. Opolon, Y. Lecluse, D. Grunenwald, M. Kubin, J.-C. Soria, S. Chouaib, and F. Mami-Chouaib
NK Cells Infiltrating a MHC Class I-Deficient Lung Adenocarcinoma Display Impaired Cytotoxic Activity toward Autologous Tumor Cells Associated with Altered NK Cell-Triggering Receptors
J. Immunol., November 1, 2005; 175(9): 5790 - 5798.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
G. Dorothee, I. Vergnon, F. El Hage, B. L. M. Chansac, V. Ferrand, Y. Lecluse, P. Opolon, S. Chouaib, G. Bismuth, and F. Mami-Chouaib
In Situ Sensory Adaptation of Tumor-Infiltrating T Lymphocytes to Peptide-MHC Levels Elicits Strong Antitumor Reactivity
J. Immunol., June 1, 2005; 174(11): 6888 - 6897.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
J. Thiery, S. Abouzahr, G. Dorothee, A. Jalil, C. Richon, I. Vergnon, F. Mami-Chouaib, and S. Chouaib
p53 Potentiation of Tumor Cell Susceptibility to CTL Involves Fas and Mitochondrial Pathways
J. Immunol., January 15, 2005; 174(2): 871 - 878.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
A. Ladanyi, B. Somlai, K. Gilde, Z. Fejos, I. Gaudi, and J. Timar
T-Cell Activation Marker Expression on Tumor-Infiltrating Lymphocytes As Prognostic Factor in Cutaneous Malignant Melanoma
Clin. Cancer Res., January 15, 2004; 10(2): 521 - 530.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
V. Dangles-Marie, S. Richon, M. El Behi, H. Echchakir, G. Dorothee, J. Thiery, P. Validire, I. Vergnon, J. Menez, M. Ladjimi, et al.
A Three-Dimensional Tumor Cell Defect in Activating Autologous CTLs Is Associated with Inefficient Antigen Presentation Correlated with Heat Shock Protein-70 Down-Regulation
Cancer Res., July 1, 2003; 63(13): 3682 - 3687.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
J. Thiery, G. Dorothee, H. Haddada, H. Echchakir, C. Richon, R. Stancou, I. Vergnon, J. Benard, F. Mami-Chouaib, and S. Chouaib
Potentiation of a Tumor Cell Susceptibility to Autologous CTL Killing by Restoration of Wild-Type p53 Function
J. Immunol., June 15, 2003; 170(12): 5919 - 5926.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
G. Dorothee, I. Vergnon, J. Menez, H. Echchakir, D. Grunenwald, M. Kubin, S. Chouaib, and F. Mami-Chouaib
Tumor-Infiltrating CD4+ T Lymphocytes Express APO2 Ligand (APO2L)/TRAIL upon Specific Stimulation with Autologous Lung Carcinoma Cells: Role of IFN-{alpha} on APO2L/TRAIL Expression and -Mediated Cytotoxicity
J. Immunol., July 15, 2002; 169(2): 809 - 817.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
H. Echchakir, G. Dorothee, I. Vergnon, J. Menez, S. Chouaib, and F. Mami-Chouaib
Cytotoxic T lymphocytes directed against a tumor-specific mutated antigen display similar HLA tetramer binding but distinct functional avidity and tissue distribution
PNAS, July 9, 2002; 99(14): 9358 - 9363.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
H. Echchakir, F. Mami-Chouaib, I. Vergnon, J.-F. Baurain, V. Karanikas, S. Chouaib, and P. G. Coulie
A Point Mutation in the {{alpha}}-Actinin-4 Gene Generates an Antigenic Peptide Recognized by Autologous Cytolytic T Lymphocytes on a Human Lung Carcinoma
Cancer Res., May 1, 2001; 61(10): 4078 - 4083.
[Abstract] [Full Text]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.