International Immunology, Vol. 12, No. 2, 195-203,
February 2000
© 2000 Japanese Society for Immunology
Soluble human MHC class I molecules induce soluble Fas ligand secretion and trigger apoptosis in activated CD8+ Fas (CD95)+ T lymphocytes
Department of Internal Medicine, University of Genoa, Viale Benedetto XV no. 6, 16132 Genoa, Italy
1 Department of Immunology, Roswell Park Cancer Institute, Buffalo, NY 14263, USA
Correspondence to: F. Puppo
In the present study, we have evaluated the apoptotic effect of soluble human MHC class I (sHLA-I) antigens on CD8+ T lymphocytes. sHLA-I antigens and ß2-microglobulin-free HLA class I heavy chains, isolated from serum, induced apoptosis on phytohemagglutinin-activated CD8+ T lymphocytes in autologous and allogeneic combinations. The extent of CD8+ T cell apoptosis depends on the degree of activation, time of incubation with sHLA-I antigens and amount of sHLA-I antigens added to the cultures. Apoptosis is induced by the interaction of Fas (CD95)+ cells with soluble Fas ligand which is released following binding of sHLA-I antigens to CD8 molecules. These results suggest that sHLA-I antigens may regulate immune responses by inducing apoptosis in activated CD8+ T cells.
Keywords: HLA, immunomodulation
Transmitting editor: L. Moretta
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