Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (33)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Radu, C. G.
Right arrow Articles by Ward, E. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Radu, C. G.
Right arrow Articles by Ward, E. S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

International Immunology, Vol. 12, No. 11, 1553-1560, November 2000
© 2000 Japanese Society for Immunology

Detection of autoreactive T cells in H-2u mice using peptide–MHC multimers

Caius G. Radu, Stephen M. Anderton1, Mihail Firan, David C. Wraith1 and E. Sally Ward

Center for Immunology and Cancer Immunobiology Center, University of Texas Southwestern Medical Center, Dallas, TX 75235-8576, USA
1 Department of Pathology and Microbiology, University of Bristol, Bristol BS8 1TD, UK

Correspondence to: E. S. Ward

Myelin basic protein (MBP)-specific T cells play a critical role in the pathogenesis of experimental autoimmune encephalomyelitis (EAE), a prototype for T cell-mediated autoimmunity. In PL/J and B10.PL mice (H-2u haplotype), the immunodominant epitope of MBP is represented by an N-terminal nonameric peptide, MBP1–9. To date, the MBP1–9-specific T cell repertoire has not been analyzed in quantitative terms. In the present study we demonstrate, using MHC class II tetramers, that 15,000–70,000 self-antigen-specific Th cells accumulate in the draining lymph nodes following immunization with spinal cord homogenate or MBP1–9. In contrast, MBP1–9-specific T cells are undetectable in unimmunized H-2u mice and represent >60% of the CD4 cells in naive mice transgenic for a TCR specific for this epitope. The results suggest that the extremely low affinity of the N-terminal peptide for I-Au does not limit the MBP1–9-specific T cells from expanding into a sizeable pool of autoreactive T cells. Therefore, the primary immune response to MBP1–9 does not differ quantitatively from previously reported CD4+ T cell responses to foreign antigens.

Keywords: autoimmunity, clonal expansion, experimental autoimmune encephalomyelitis, MHC class II tetramers, T lymphocytes

Transmitting editor: C. Martinez-A


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
P. Prabhat, Z. Gan, J. Chao, S. Ram, C. Vaccaro, S. Gibbons, R. J. Ober, and E. S. Ward
Elucidation of intracellular recycling pathways leading to exocytosis of the Fc receptor, FcRn, by using multifocal plane microscopy
PNAS, April 3, 2007; 104(14): 5889 - 5894.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. Minguela, S. Pastor, W. Mi, J. A. Richardson, and E. S. Ward
Feedback Regulation of Murine Autoimmunity via Dominant Anti-Inflammatory Effects of Interferon {gamma}
J. Immunol., January 1, 2007; 178(1): 134 - 144.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
S. Pastor, C. G. Vaccaro, A. Minguela, R. J. Ober, and E. S. Ward
Analyses of TCR clustering at the T cell-antigen-presenting cell interface and its impact on the activation of naive CD4+ T cells
Int. Immunol., November 1, 2006; 18(11): 1615 - 1625.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
F. C. Kurschus, T. Oelert, B. Liliensiek, P. Buchmann, D. C. Wraith, G. J. Hammerling, and B. Arnold
Experimental autoimmune encephalomyelitis in mice expressing the autoantigen MBP1-10 covalently bound to the MHC class II molecule I-Au
Int. Immunol., January 1, 2006; 18(1): 151 - 162.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
K. R. Ryan, D. McCue, and S. M. Anderton
Fas-mediated death and sensory adaptation limit the pathogenic potential of autoreactive T cells after strong antigenic stimulation
J. Leukoc. Biol., July 1, 2005; 78(1): 43 - 50.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
H. Reijonen, R. Mallone, A.-K. Heninger, E. M. Laughlin, S. A. Kochik, B. Falk, W. W. Kwok, C. Greenbaum, and G. T. Nepom
GAD65-Specific CD4+ T-Cells with High Antigen Avidity Are Prevalent in Peripheral Blood of Patients With Type 1 Diabetes
Diabetes, August 1, 2004; 53(8): 1987 - 1994.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
K. L. Laurie, N. L. La Gruta, N. Koch, I. R. van Driel, and P. A. Gleeson
Thymic Expression of a Gastritogenic Epitope Results in Positive Selection of Self-Reactive Pathogenic T Cells
J. Immunol., May 15, 2004; 172(10): 5994 - 6002.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
J. C. Huang, M. Vestberg, A. Minguela, R. Holmdahl, and E. S. Ward
Analysis of autoreactive T cells associated with murine collagen-induced arthritis using peptide-MHC multimers
Int. Immunol., February 1, 2004; 16(2): 283 - 293.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
J. C. Huang, M. Han, A. Minguela, S. Pastor, A. Qadri, and E. S. Ward
T Cell Recognition of Distinct Peptide:I-Au Conformers in Murine Experimental Autoimmune Encephalomyelitis
J. Immunol., September 1, 2003; 171(5): 2467 - 2477.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
D. Sun, Y. Zhang, B. Wei, S. C. Peiper, H. Shao, and H. J. Kaplan
Encephalitogenic activity of truncated myelin oligodendrocyte glycoprotein (MOG) peptides and their recognition by CD8+ MOG-specific T cells on oligomeric MHC class I molecules
Int. Immunol., February 1, 2003; 15(2): 261 - 268.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
J. Reddy, E. Bettelli, L. Nicholson, H. Waldner, M.-H. Jang, K. W. Wucherpfennig, and V. K. Kuchroo
Detection of Autoreactive Myelin Proteolipid Protein 139-151-Specific T Cells by Using MHC II (IAs) Tetramers
J. Immunol., January 15, 2003; 170(2): 870 - 877.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
C. Deng, A. Minguela, R. Z. Hussain, A. E. Lovett-Racke, C. Radu, E. S. Ward, and M. K. Racke
Expression of the Tyrosine Phosphatase Src Homology 2 Domain-Containing Protein Tyrosine Phosphatase 1 Determines T Cell Activation Threshold and Severity of Experimental Autoimmune Encephalomyelitis
J. Immunol., May 1, 2002; 168(9): 4511 - 4518.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
H. Quarsten, S. N. McAdam, T. Jensen, H. Arentz-Hansen, K. E. A. Lundin, and L. M. Sollid
Staining of Celiac Disease-Relevant T Cells by Peptide-DQ2 Multimers
J. Immunol., November 1, 2001; 167(9): 4861 - 4868.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
K. C. Garcia, C. G. Radu, J. Ho, R. J. Ober, and E. S. Ward
Kinetics and thermodynamics of T cell receptor- autoantigen interactions in murine experimental autoimmune encephalomyelitis
PNAS, June 5, 2001; 98(12): 6818 - 6823.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Med.Home page
S. M. Anderton, C. G. Radu, P. A. Lowrey, E. S. Ward, and D. C. Wraith
Negative Selection during the Peripheral Immune Response to Antigen
J. Exp. Med., December 27, 2000; 193(1): 1 - 12.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.