Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (11)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Yang, W.-C.
Right arrow Articles by Olive, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yang, W.-C.
Right arrow Articles by Olive, D.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

International Immunology, Vol. 12, No. 11, 1547-1552, November 2000
© 2000 Japanese Society for Immunology

Role of Tec kinase in nuclear factor of activated T cells signaling

Wen-Chin Yang2, Marguerite Ghiotto, Rémy Castellano, Yves Collette, Nathalie Auphan1, Jacques A. Nunès and Daniel Olive

INSERM U119, Institut d'Immunologie et de Cancérologie de Marseille, Université de la Méditerranée, 27 Bd Leï Roure, 13009 Marseille, France
1 Centre d'Immunologie INSERM-CNRS de Marseille-Luminy, 13288 Marseille, France

Correspondence to: J. A. Nunès or D. Olive

The Tec protein kinase family includes Btk, Itk, Tec, Rlk and Bmx, which are critically involved in signals mediated by various cytokines and antigen receptors. Btk mutations cause severe immunodeficiencies, with defective B cell function. In T cells, Tec regulates cytokine production. However, the downstream targets of these Tec kinases are poorly defined. Here we report that overexpression of Tec in T cells can regulate gene transcription through the nuclear factor of activated T cells (NF-AT). Using different reporter gene constructs, we establish that Tec in transfected T cells dramatically induced NF-AT-dependent gene transcription, which was prevented by a dominant-negative mutant of NF-AT or by the immunosuppressive drug cyclosporin A. Tec appears to regulate NF-AT nuclear import. In addition, Tec influences cytoplasmic free calcium increase. Taken together, our results identify NF-AT as a major downstream target of Tec kinases that is critically involved in transcriptional gene regulation. These observations highlight signaling pathways regulated by Tec kinases and provide new pharmacological targets to regulate immune functions.

Keywords: signal transduction, T cells, Tec family kinases, transcription factors

2 Present address: Department of Pathology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA

Transmitting editor: L. Moretta


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
L. P. Kane and S. C. Watkins
Dynamic Regulation of Tec Kinase Localization in Membrane-proximal Vesicles of a T Cell Clone Revealed by Total Internal Reflection Fluorescence and Confocal Microscopy
J. Biol. Chem., June 10, 2005; 280(23): 21949 - 21954.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Med.Home page
T. J. Hagenbeek, M. Naspetti, F. Malergue, F. Garcon, J. A. Nunes, K. B.J.M. Cleutjens, J. Trapman, P. Krimpenfort, and H. Spits
The Loss of PTEN Allows TCR {alpha}{beta} Lineage Thymocytes to Bypass IL-7 and Pre-TCR-mediated Signaling
J. Exp. Med., October 4, 2004; 200(7): 883 - 894.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
F. Garcon, G. Bismuth, D. Isnardon, D. Olive, and J. A. Nunes
Tec Kinase Migrates to the T Cell-APC Interface Independently of Its Pleckstrin Homology Domain
J. Immunol., July 15, 2004; 173(2): 770 - 775.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
M. Hirano, Y. Kikuchi, S. Nisitani, A. Yamaguchi, A. Satoh, T. Ito, H. Iba, and K. Takatsu
Bruton's tyrosine kinase (Btk) enhances transcriptional co-activation activity of BAM11, a Btk-associated molecule of a subunit of SWI/SNF complexes
Int. Immunol., May 1, 2004; 16(5): 747 - 757.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
M. G. Tomlinson, L. P. Kane, J. Su, T. A. Kadlecek, M. N. Mollenauer, and A. Weiss
Expression and Function of Tec, Itk, and Btk in Lymphocytes: Evidence for a Unique Role for Tec
Mol. Cell. Biol., March 15, 2004; 24(6): 2455 - 2466.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Med.Home page
C.-H. Chen, C. Seguin-Devaux, N. A. Burke, T. B. Oriss, S. C. Watkins, N. Clipstone, and A. Ray
Transforming Growth Factor {beta} Blocks Tec Kinase Phosphorylation, Ca2+ Influx, and NFATc Translocation Causing Inhibition of T Cell Differentiation
J. Exp. Med., June 16, 2003; 197(12): 1689 - 1699.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. Castellano, C. Van Lint, V. Peri, E. Veithen, Y. Morel, R. Costello, D. Olive, and Y. Collette
Mechanisms Regulating Expression of the Tumor Necrosis Factor-related light Gene. ROLE OF CALCIUM-SIGNALING PATHWAY IN THE TRANSCRIPTIONAL CONTROL
J. Biol. Chem., November 1, 2002; 277(45): 42841 - 42851.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.