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International Immunology, Vol. 11, No. 9, 1423-1429, September 1999
© 1999 Japanese Society for Immunology

Prf, a novel Ets family protein that binds to the PU.1 binding motif, is specifically expressed in restricted stages of B cell development

Shu-ichi Hashimoto, Hirofumi Nishizumi, Reiko Hayashi, Akio Tsuboi, Fumikiyo Nagawa, Toshitada Takemori1 and Hitoshi Sakano

Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan
1 Department of Immunology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan

Correspondence to: H. Sakano

During the development of lymphocytes, expression of the Ig genes is strictly regulated in a tissue-specific manner and in a time-ordered fashion. We have previously shown that the PU.1 binding motif in the Ig{kappa} 3' enhancer ({kappa}E3') and a novel Ets family protein other than PU.1 may be possibly involved in the control of V{kappa}–J{kappa} joining. In the attempt to isolate the novel Ets family protein, we have screened cDNA libraries with the yeast one-hybrid method and identified a new PU.1-related factor, Prf. This novel Ets family protein is shown to interact with the PU.1 binding sequences in various promoters and enhancers, including {kappa}E3'. It was found that expression of the prf gene is predominant in the B-lineage cells, with the exception of immature B cells. Since Prf does not exhibit functions of transcriptional activity, this novel protein may act as an antagonist against other Ets family proteins, e.g. PU.1 and Spi-B. Possible roles of Prf with respect to the B cell differentiation are discussed.

Keywords: 3' enhancer, B cell development, Ets family protein, Ig{kappa} gene, PU.1

Transmitting editor: K. Okumura


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