International Immunology, Vol. 11, No. 7, 1149-1156,
July 1999
© 1999 Japanese Society for Immunology
Target cells for an immunosuppressive cytokine, glycosylation-inhibiting factor
1 Division of Immunobiology and
2 Division of Immunochemistry, La Jolla Institute for Allergy and Immunology, San Diego, CA 92121, USA
3 Kirin Pharmaceutical Laboratory, Takasaki 370-12, Japan
4 Department of Obstetrics and Gynecology, Shiga University of Medical Science, Shiga 520-21, Japan
5 Division of Molecular Immunology, Center for Biomedical Science, Chiba University School of Medicine, Chiba 260, Japan
Correspondence to: K. Ishizaka
Receptors for bioactive glycosylation-inhibiting factor (GIF) were demonstrated using a bioactive mutant of recombinant human (rh) GIF, which is comparable to the suppressor T (Ts) cell-derived bioactive GIF in its affinity for the receptors on helper T (Th) hybridoma cells. Both naive T and B cells in normal mouse spleen lacked GIF receptors. However, presentation of specific antigen to naive T cells resulted in the expression of the receptors on activated T cells. Furthermore, activation of small resting B cells with F(ab')2 fragments of anti-mouse IgM plus IL-4, lipopolysaccharide (LPS) plus IL-4 or LPS plus dextran sulfate induced the expression of the receptors within 48 h of B cell stimulation. It was also found that NK T cells freshly isolated from mouse spleen, but not conventional NK cells, expressed receptors for GIF. CD4+ and CD4– subpopulations of NK T cells showed a similar binding capability. Mature dendritic cells derived from bone marrow did not bear the receptors. The dissociation constant (Kd) of the interaction between the bioactive rhGIF mutant and the high-affinity receptors was 10–100 pM, whereas inactive wild-type rhGIF failed to bind to the receptors. A bioactive derivative of rhGIF suppressed both IgG1 and IgE synthesis by purified B cells activated by LPS and IL-4, indicating that the binding of bioactive GIF to its receptors on activated B cells results in suppression of their differentiation.
Keywords: cytokine receptor, Ig
Transmitting editor: T. Sasazuki
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  A. Kudrin, M. Scott, S. Martin, C.-w. Chung, R. Donn, A. McMaster, S. Ellison, D. Ray, K. Ray, and M. Binks Human Macrophage Migration Inhibitory Factor: A PROVEN IMMUNOMODULATORY CYTOKINE? J. Biol. Chem., October 6, 2006; 281(40): 29641 - 29651. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Sugie and J. Huang GIF Inhibits Th Effector Generation by Acting on Antigen-Presenting B Cells J. Immunol., April 1, 2001; 166(7): 4473 - 4480. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Watarai, R. Nozawa, A. Tokunaga, N. Yuyama, M. Tomas, A. Hinohara, K. Ishizaka, and Y. Ishii Posttranslational modification of the glycosylation inhibiting factor (GIF) gene product generates bioactive GIF PNAS, November 2, 2000; (2000) 230445397. [Abstract] [Full Text]
|
|
|
|
 |
|
 K. Kasahara, T. Nakano, H. Takahashi, Y. Ishii, K. Ishizaka, and K. Imai Presence of the 55 kDa glycosylation inhibiting factor in human serum Int. Immunol., September 1, 2000; 12(9): 1303 - 1309. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Watarai, R. Nozawa, A. Tokunaga, N. Yuyama, M. Tomas, A. Hinohara, K. Ishizaka, and Y. Ishii Posttranslational modification of the glycosylation inhibiting factor (GIF) gene product generates bioactive GIF PNAS, November 21, 2000; 97(24): 13251 - 13256. [Abstract] [Full Text] [PDF]
|
|