Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (78)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Martin-Orozco, E.
Right arrow Articles by Raz, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Martin-Orozco, E.
Right arrow Articles by Raz, E.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

International Immunology, Vol. 11, No. 7, 1111-1118, July 1999
© 1999 Japanese Society for Immunology

Enhancement of antigen-presenting cell surface molecules involved in cognate interactions by immunostimulatory DNA sequences

Elena Martin-Orozco, Hiroko Kobayashi, John Van Uden, Minh-Duc Nguyen, Richard S. Kornbluth and Eyal Raz

Department of Medicine, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA

Correspondence to: E. Raz

Bacterial genomic DNA, plasmid DNA (pDNA) and synthetic oligodeoxynucleotides (ODN) containing immunostimulatory DNA sequences (ISS) have been proposed to foster a Th1 response via the release of type 1 cytokines from macrophages, dendritic cells, NK cells and B cells. In this study, we show that ISS-enriched DNA up-regulates a distinct profile of cell surface molecules on macrophages and B cells in vitro and in vivo. ISS-ODN and ISS-containing pDNA enhanced the expression of antigen presentation molecules (MHC class I and II), co-stimulatory molecules (B7-1, B7-2 and CD40), cytokine receptors (IFN-{gamma} receptor and IL-2 receptor), an adhesion molecule (ICAM-1) and an Fc receptor (Fc{gamma} receptor) on murine B cells or bone marrow-derived macrophages. The increased expression of these surface molecules is seen in purified cell populations and is largely independent of the effects of type 1 cytokines. Splenic antigen-presenting cells stimulated with ISS-ODN in vivo efficiently activate naive T cells and bias their differentiation toward a Th1 phenotype in vitro . Thus, the induction of both type 1 cytokines and a distinct profile of cell surface molecules contributes to the potent immunostimulatory effects of ISS-containing DNA on innate and adaptive immunity.

Keywords: activation markers, antigen-presenting cells, CpG motifs, immunostimulatory DNA sequences, Th1 response

The first two authors contributed equally to this work

Transmitting editor: K. Okumura


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
K. Abe, K. P. Nguyen, S. D. Fine, J.-H. Mo, C. Shen, S. Shenouda, M. Corr, S. Jung, J. Lee, L. Eckmann, et al.
Conventional dendritic cells regulate the outcome of colonic inflammation independently of T cells
PNAS, October 23, 2007; 104(43): 17022 - 17027.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
K. J. Serio, C. Luo, L. Luo, and J. T. Mao
TNF-{alpha} downregulates the leukotriene C4 synthase gene in mononuclear phagocytes
Am J Physiol Lung Cell Mol Physiol, January 1, 2007; 292(1): L215 - L222.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
J. N. Radcliffe, J. S. Roddick, P. S. Friedmann, F. K. Stevenson, and S. M. Thirdborough
Prime-Boost with Alternating DNA Vaccines Designed to Engage Different Antigen Presentation Pathways Generates High Frequencies of Peptide-Specific CD8+ T Cells
J. Immunol., November 15, 2006; 177(10): 6626 - 6633.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
C. J. Youn, M. Miller, K. J. Baek, J. W. Han, J. Nayar, S. Y. Lee, K. McElwain, S. McElwain, E. Raz, and D. H. Broide
Immunostimulatory DNA Reverses Established Allergen-Induced Airway Remodeling
J. Immunol., December 15, 2004; 173(12): 7556 - 7564.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. B. Rosset, C. Ballerini, S. Gregoire, P. Metharom, C. Carnaud, and P. Aucouturier
Breaking Immune Tolerance to the Prion Protein Using Prion Protein Peptides Plus Oligodeoxynucleotide-CpG in Mice
J. Immunol., May 1, 2004; 172(9): 5168 - 5174.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
C. S. Goodyear, M. Narita, and G. J. Silverman
In Vivo VL-Targeted Activation-Induced Apoptotic Supraclonal Deletion by a Microbial B Cell Toxin
J. Immunol., March 1, 2004; 172(5): 2870 - 2877.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
P. P. Ho, P. Fontoura, P. J. Ruiz, L. Steinman, and H. Garren
An Immunomodulatory GpG Oligonucleotide for the Treatment of Autoimmunity via the Innate and Adaptive Immune Systems
J. Immunol., November 1, 2003; 171(9): 4920 - 4926.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. H. Manjili, X.-Y. Wang, X. Chen, T. Martin, E. A. Repasky, R. Henderson, and J. R. Subjeck
HSP110-HER2/neu Chaperone Complex Vaccine Induces Protective Immunity Against Spontaneous Mammary Tumors in HER-2/neu Transgenic Mice
J. Immunol., October 15, 2003; 171(8): 4054 - 4061.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. D. Marshall, E. M. Hessel, J. Gregorio, C. Abbate, P. Yee, M. Chu, G. V. Nest, R. L. Coffman, and K. L. Fearon
Novel chimeric immunomodulatory compounds containing short CpG oligodeoxyribonucleotides have differential activities in human cells
Nucleic Acids Res., September 1, 2003; 31(17): 5122 - 5133.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
R. Rieger and T. J. Kipps
CpG Oligodeoxynucleotides Enhance the Capacity of Adenovirus-mediated CD154 Gene Transfer to Generate Effective B-Cell Lymphoma Vaccines
Cancer Res., July 15, 2003; 63(14): 4128 - 4135.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
C. S. Goodyear and G. J. Silverman
Death by a B Cell Superantigen: In Vivo VH-targeted Apoptotic Supraclonal B Cell Deletion by a Staphylococcal Toxin
J. Exp. Med., May 5, 2003; 197(9): 1125 - 1139.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. K. Datta, V. Redecke, K. R. Prilliman, K. Takabayashi, M. Corr, T. Tallant, J. DiDonato, R. Dziarski, S. Akira, S. P. Schoenberger, et al.
A Subset of Toll-Like Receptor Ligands Induces Cross-presentation by Bone Marrow-Derived Dendritic Cells
J. Immunol., April 15, 2003; 170(8): 4102 - 4110.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
M. Gierynska, U. Kumaraguru, S.-K. Eo, S. Lee, A. Krieg, and B. T. Rouse
Induction of CD8 T-Cell-Specific Systemic and Mucosal Immunity against Herpes Simplex Virus with CpG-Peptide Complexes
J. Virol., June 5, 2002; 76(13): 6568 - 6576.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
H. J. Cho, T. Hayashi, S. K. Datta, K. Takabayashi, J. H. Van Uden, A. Horner, M. Corr, and E. Raz
IFN-{alpha}{beta} Promote Priming of Antigen-Specific CD8+ and CD4+ T Lymphocytes by Immunostimulatory DNA-Based Vaccines
J. Immunol., May 15, 2002; 168(10): 4907 - 4913.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. Ronaghy, B. J. Prakken, K. Takabayashi, G. S. Firestein, D. Boyle, N. J. Zvailfler, S. T. A. Roord, S. Albani, D. A. Carson, and E. Raz
Immunostimulatory DNA Sequences Influence the Course of Adjuvant Arthritis
J. Immunol., January 1, 2002; 168(1): 51 - 56.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
T. Hayashi, S. P. Rao, K. Takabayashi, J. H. Van Uden, R. S. Kornbluth, S. M. Baird, M. W. Taylor, D. A. Carson, A. Catanzaro, and E. Raz
Enhancement of Innate Immunity against Mycobacterium avium Infection by Immunostimulatory DNA Is Mediated by Indoleamine 2,3-Dioxygenase
Infect. Immun., October 1, 2001; 69(10): 6156 - 6164.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. A. Horner, S. K. Datta, K. Takabayashi, I. M. Belyakov, T. Hayashi, N. Cinman, M.-D. Nguyen, J. H. Van Uden, J. A. Berzofsky, D. D. Richman, et al.
Immunostimulatory DNA-Based Vaccines Elicit Multifaceted Immune Responses Against HIV at Systemic and Mucosal Sites
J. Immunol., August 1, 2001; 167(3): 1584 - 1591.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
L. K. M. Shoda, K. A. Kegerreis, C. E. Suarez, W. Mwangi, D. P. Knowles, and W. C. Brown
Immunostimulatory CpG-modified plasmid DNA enhances IL-12, TNF-{alpha}, and NO production by bovine macrophages
J. Leukoc. Biol., July 1, 2001; 70(1): 103 - 112.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
D. Miyazaki, G. Liu, L. Clark, and S. J. Ono
Prevention of Acute Allergic Conjunctivitis and Late-Phase Inflammation with Immunostimulatory DNA Sequences
Invest. Ophthalmol. Vis. Sci., November 1, 2000; 41(12): 3850 - 3855.
[Abstract] [Full Text]

Home page
 J. Immunol.Home page
B. M. Segal, J. T. Chang, and E. M. Shevach
CpG Oligonucleotides Are Potent Adjuvants for the Activation of Autoreactive Encephalitogenic T Cells In Vivo
J. Immunol., June 1, 2000; 164(11): 5683 - 5688.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
C. von Hunolstein, R. Teloni, S. Mariotti, S. Recchia, G. Orefici, and R. Nisini
Synthetic oligodeoxynucleotide containing CpG motif induces an anti-polysaccharide type 1-like immune response after immunization of mice with Haemophilus influenzae type b conjugate vaccine
Int. Immunol., March 1, 2000; 12(3): 295 - 303.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.