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International Immunology, Vol. 11, No. 7, 1093-1102, July 1999
© 1999 Japanese Society for Immunology

Adjuvants that enhance priming of cytotoxic T cells to a Kb-restricted epitope processed from exogenous but not endogenous hepatitis B surface antigen

Reinhold Schirmbeck, Karl Melber1 and Jörg Reimann

Institute for Medical Microbiology and Immunology, University of Ulm, Helmholtzstrasse 8/1, 89081, Ulm, Germany
1 Rhein-Biotech GmbH, Eichsfelderstr. 11, 40595 Düsseldorf, Germany

Correspondence to: J. Reimann

Intramuscular (i.m.) or s.c. injection of plasmid DNA encoding hepatitis B small surface antigen (HBsAg) primes potent MHC I-restricted cytotoxic T lymphocyte (CTL) responses in H-2d (BALB/c) and H-2b (C57BL/6) mice. In contrast, i.m. or s.c. injection of exogenous HBsAg particles without adjuvants primes CTL responses in `high responder' H-2d but not `low responder' H-2b mice. We have shown that processing of exogenous but not endogenous HBsAg generates the Kb-binding S208–215 peptide ILSPFLPL. This system allowed us to optimize conditions for stimulating murine CTL responses to exogenous antigen by identifying adjuvants that facilitate priming of Kb-restricted CTL by injecting recombinant HBsAg particles into `low responder' H-2b mice. Synthetic oligodeoxynucleotides with immunostimulating sequences or the recombinant cytokine IL-12 efficiently enhanced priming of CTL to exogenous HBsAg. Hence, the adjuvanticity of DNA sequences that induce Th1 cytokines facilitate priming of MHC I-restricted T cell responses to exogenous antigen and are therefore of potential value in formulating vaccines designed to enhance CTL priming to exogenous antigen.

Keywords: cytotoxic T lymphocyte, hepatitis B surface antigen, MHC class I, mouse

Transmitting editor: S. H. Kaufmann


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