Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (43)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Kawano, T.
Right arrow Articles by Taniguchi, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kawano, T.
Right arrow Articles by Taniguchi, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

International Immunology, Vol. 11, No. 6, 881-887, June 1999
© 1999 Japanese Society for Immunology

A novel recognition motif of human NKT antigen receptor for a glycolipid ligand

Tetsu Kawano, Yujiro Tanaka1, Eiko Shimizu, Yoshikatsu Kaneko, Noriaki Kamata, Hiroshi Sato, Hisao Osada2, Soei Sekiya2, Toshinori Nakayama and Masaru Taniguchi

CREST (Core Research for Evolutional Science and Technology) Project and Department of Molecular Immunology,
1 Department of Developmental Immunology, Graduate School of Medicine, and
2 Department of Obstetrics and Gynaecology, School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8670, Japan

Correspondence to: M Taniguchi

Murine NKT cells can recognize {alpha}-galactosylceramide ({alpha}-GalCer) in the context of a class Ib CD1d molecule. Here we show that {alpha}-GalCer can selectively activate freshly isolated human V{alpha}24+Vß11+ cells, functionally defining the human NKT cells. The naive human NKT cell repertoire consisted of cells expressing an invariant V{alpha}24J{alpha}Q chain and a diverse array of ß chains derived from a single Vß11 gene segment. Stimulation with {alpha}-GalCer expanded a polyclonal subset of the human NKT cell repertoire carrying a novel complementarity-determining region (CDR) 3ß consensus motif that may directly interact with the sugar moiety of {alpha}-GalCer. Our data suggest that certain redundancy is allowed for CDR3ß of NKT antigen receptor to interact with the ligand and provide a first clue to understand the novel protein–carbohydrate interaction mechanisms.

Keywords: carbohydrate-binding proteins, complementarity-determining region, NKT antigen receptor, protein–sugar interaction, repertoire selection

Transmitting editor: T. Watanabe


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 JEMHome page
L. Kjer-Nielsen, N. A. Borg, D. G. Pellicci, T. Beddoe, L. Kostenko, C. S. Clements, N. A. Williamson, M. J. Smyth, G. S. Besra, H. H. Reid, et al.
A structural basis for selection and cross-species reactivity of the semi-invariant NKT cell receptor in CD1d/glycolipid recognition
J. Exp. Med., March 20, 2006; 203(3): 661 - 673.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
A. Ishikawa, S. Motohashi, E. Ishikawa, H. Fuchida, K. Higashino, M. Otsuji, T. Iizasa, T. Nakayama, M. Taniguchi, and T. Fujisawa
A Phase I Study of {alpha}-Galactosylceramide (KRN7000)-Pulsed Dendritic Cells in Patients with Advanced and Recurrent Non-Small Cell Lung Cancer
Clin. Cancer Res., March 1, 2005; 11(5): 1910 - 1917.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
T. Crough, M. Nieda, and A. J. Nicol
Granulocyte Colony-Stimulating Factor Modulates {alpha}-Galactosylceramide-Responsive Human V{alpha}24+V{beta}11+ NKT Cells
J. Immunol., October 15, 2004; 173(8): 4960 - 4966.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
M. Skold and S. M. Behar
Role of CD1d-Restricted NKT Cells in Microbial Immunity
Infect. Immun., October 1, 2003; 71(10): 5447 - 5455.
[Full Text] [PDF]

Home page
 J. Immunol.Home page
B. Gansuvd, W. J. Hubbard, A. Hutchings, F. T. Thomas, J. Goodwin, S. B. Wilson, M. A. Exley, and J. M. Thomas
Phenotypic and Functional Characterization of Long-Term Cultured Rhesus Macaque Spleen-Derived NKT Cells
J. Immunol., September 15, 2003; 171(6): 2904 - 2911.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
T. Ulrichs, D. B. Moody, E. Grant, S. H. E. Kaufmann, and S. A. Porcelli
T-Cell Responses to CD1-Presented Lipid Antigens in Humans with Mycobacterium tuberculosis Infection
Infect. Immun., June 1, 2003; 71(6): 3076 - 3087.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
K. Yanagisawa, K.-i. Seino, Y. Ishikawa, M. Nozue, T. Todoroki, and K. Fukao
Impaired Proliferative Response of V{alpha}24 NKT Cells from Cancer Patients Against {alpha}-Galactosylceramide
J. Immunol., June 15, 2002; 168(12): 6494 - 6499.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
L. S. Metelitsa, O. V. Naidenko, A. Kant, H.-W. Wu, M. J. Loza, B. Perussia, M. Kronenberg, and R. C. Seeger
Human NKT Cells Mediate Antitumor Cytotoxicity Directly by Recognizing Target Cell CD1d with Bound Ligand or Indirectly by Producing IL-2 to Activate NK Cells
J. Immunol., September 15, 2001; 167(6): 3114 - 3122.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
N. Kamada, H. Iijima, K. Kimura, M. Harada, E. Shimizu, S.-i. Motohashi, T. Kawano, H. Shinkai, T. Nakayama, T. Sakai, et al.
Crucial amino acid residues of mouse CD1d for glycolipid ligand presentation to V{{alpha}}14 NKT cells
Int. Immunol., July 1, 2001; 13(7): 853 - 861.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
N. Kadowaki, S. Antonenko, S. Ho, M.-C. Rissoan, V. Soumelis, S. A. Porcelli, L. L. Lanier, and Y.-J. Liu
Distinct Cytokine Profiles of Neonatal Natural Killer T Cells after Expansion with Subsets of Dendritic Cells
J. Exp. Med., May 21, 2001; 193(10): 1221 - 1226.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
C. Ronet, M. Mempel, N. Thieblemont, A. Lehuen, P. Kourilsky, and G. Gachelin
Role of the Complementarity-Determining Region 3 (CDR3) of the TCR-{{beta}} Chains Associated with the V{{alpha}}14 Semi-Invariant TCR {{alpha}}-Chain in the Selection of CD4+ NK T Cells
J. Immunol., February 1, 2001; 166(3): 1755 - 1762.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
T. J. Sayers, A. D. Brooks, J. M. Ward, T. Hoshino, W. E. Bere, G. W. Wiegand, J. M. Kelley, and M. J. Smyth
The Restricted Expression of Granzyme M in Human Lymphocytes
J. Immunol., January 15, 2001; 166(2): 765 - 771.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
N. Burdin, L. Brossay, M. Degano, H. Iijima, M. Gui, I. A. Wilson, and M. Kronenberg
Structural requirements for antigen presentation by mouse CD1
PNAS, August 29, 2000; 97(18): 10156 - 10161.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. Ishihara, M. Nieda, J. Kitayama, T. Osada, T. Yabe, A. Kikuchi, Y. Koezuka, S. A. Porcelli, K. Tadokoro, H. Nagawa, et al.
{alpha}-Glycosylceramides Enhance the Antitumor Cytotoxicity of Hepatic Lymphocytes Obtained from Cancer Patients by Activating CD3-CD56+ NK Cells In Vitro
J. Immunol., August 1, 2000; 165(3): 1659 - 1664.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
H. J. J. van der Vliet, N. Nishi, T. D. de Gruijl, B. M. E. von Blomberg, A. J. M. van den Eertwegh, H. M. Pinedo, G. Giaccone, and R. J. Scheper
Human natural killer T cells acquire a memory-activated phenotype before birth
Blood, April 1, 2000; 95(7): 2440 - 2442.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
Y. Kaneko, M. Harada, T. Kawano, M. Yamashita, Y. Shibata, F. Gejyo, T. Nakayama, and M. Taniguchi
Augmentation of V{alpha}14 Nkt Cell-Mediated Cytotoxicity by Interleukin 4 in an Autocrine Mechanism Resulting in the Development of Concanavalin a-Induced Hepatitis
J. Exp. Med., January 3, 2000; 191(1): 105 - 114.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
T. Kawano, T. Nakayama, N. Kamada, Y. Kaneko, M. Harada, N. Ogura, Y. Akutsu, S. Motohashi, T. Iizasa, H. Endo, et al.
Antitumor Cytotoxicity Mediated by Ligand-activated Human V{{alpha}}24 NKT Cells
Cancer Res., October 1, 1999; 59(20): 5102 - 5105.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
I. Toura, T. Kawano, Y. Akutsu, T. Nakayama, T. Ochiai, and M. Taniguchi
Cutting Edge: Inhibition of Experimental Tumor Metastasis by Dendritic Cells Pulsed with {alpha}-Galactosylceramide
J. Immunol., September 1, 1999; 163(5): 2387 - 2391.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. L. Matsuda, L. Gapin, N. Fazilleau, K. Warren, O. V. Naidenko, and M. Kronenberg
Natural killer T cells reactive to a single glycolipid exhibit a highly diverse T cell receptor beta repertoire and small clone size
PNAS, October 23, 2001; 98(22): 12636 - 12641.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.