International Immunology, Vol. 11, No. 4, 561-568,
April 1999
© 1999 Japanese Society for Immunology
Calcium responses elicited in human T cells and dendritic cells by cell–cell interaction and soluble ligands
Laboratoire d'Immunologie Cellulaire, UMR CNRS 7627, CERVI, 83 Boulevard de l'Hôpital, 75013 Paris France
Correspondence to: A. Trautmann
The interactions between a human CD4+ T cell clone and monocyte-derived human dendritic cells (DC) were analyzed with an imaging system. The first question addressed was the relationship between the formation of a contact zone and the triggering of a Ca2+ response in the T cells, in the presence or absence of antigen. Interaction of T cells with DC pulsed with the antigen led to the formation of a stable contact zone, followed by the appearance in the T cells of large and sustained Ca2+ oscillations. In the absence of antigen, contact zones formed normally and, surprisingly, Ca2+ responses were also observed, characterized by rare and small transients. Antigen-independent Ca2+ responses were not MHC restricted. The possible influence of Ca2+ responses in the DC on the efficiency of antigen presentation was then investigated. In DC, Ca2+ responses can be elicited by a variety of stimuli: cell adhesion, platelet-activating factor, UTP and chemotactic molecules (formyl-Met–Leu–Pro, RANTES, MIP-1ß and SDF-1
). Importantly, Ca2+ responses were also induced in ~30% of DC as a result of their interaction with T cells. However, the efficiency of antigen presentation (as judged by the percentage of T cells presenting a Ca2+ response) was independent of the Ca2+ level in DC. Thus, imaging the interactions between human T cells and DC led us to observe two novel phenomena: DC-induced but antigen-independent Ca2+ responses in T cells and T cell-induced Ca2+ responses in DC.
Keywords: calcium imaging, dendritic cells, T cells
1 Present address: Department of Genetics, University of Osaka Medical School, 2-2 Yamada-oka, Suita, Osaka 565, Japan
2 Present address: INSERM U445, Institut Cochin de Génétique Moléculaire, 27 rue du Faubourg Saint Jacques, 75014 Paris, France
Transmitting editor: J. Blanchereau
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