International Immunology, Vol. 11, No. 4, 535-543,
April 1999
© 1999 Japanese Society for Immunology
Molecular fingerprinting reveals non-overlapping T cell oligoclonality between an inflamed site and peripheral blood
Department of Molecular Pathology, University College, 46 Cleveland Street, London W1P 6DB, UK
1 ICRF Tumour Immunology Unit, University College, London W1P 8BT, UK
Correspondence to: L. R. Wedderburn
We have demonstrated a stable expansion of CD8+ T cells in the peripheral blood of a child with chronic arthritis. The expanded TCRBV family (TCRBV14) was enriched for CD57hiCD28– T cells. Sequencing of the TCRBV14 amplification products showed a TCR sequence which contributed 32% of the total TCR in the CD8+TCRBV14 population. Using the modified heteroduplex technique, the CD8+TCRBV14 cells showed a clonal pattern and these bands were restricted to the CD28– population. This method also detected multiple other clones within the CD8+ population but few in the CD4+ cells. The dominant TCRBV14+ clone was not detectable in synovial fluid T cells from two inflamed joints by CDR3 length analysis or heteroduplex probing, suggesting that this long-lived clone is excluded from inflammatory sites. Synovial fluid T cells showed an unexpected discordance of the CD28 and CD57 phenotype compared to peripheral blood mononuclear cells. T cells from both inflamed joints both showed marked oligoclonality in all TCR families and had almost identical heteroduplex patterns. Taken together these data suggest that some clones are actively excluded from inflamed sites in juvenile chronic arthritis, yet the pattern of restricted T cell expansion is shared between sites of inflammation.
Keywords: autoimmunity, clonal expansion, human, juvenile chronic arthritis, TCR
Transmitting editor: E. Simpson
2 Present address: Department of Sexually Transmitted Diseases, University College, Mortimer Market Centre, London WC1E 6AU, UK
3 Present address: The Edward Jenner Institute for Vaccine Research, Compton, Berkshire RG20 7NN, UK
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  H. Varsani, A. Patel, Y. van Kooyk, P. Woo, and L. R. Wedderburn Synovial dendritic cells in juvenile idiopathic arthritis (JIA) express receptor activator of NF-{kappa}B (RANK) Rheumatology, April 1, 2003; 42(4): 583 - 590. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. R. Wedderburn, A. Patel, H. Varsani, and P. Woo Divergence in the degree of clonal expansions in inflammatory T cell subpopulations mirrors HLA-associated risk alleles in genetically and clinically distinct subtypes of childhood arthritis Int. Immunol., December 1, 2001; 13(12): 1541 - 1550. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. R. Wedderburn, R. Jeffery, H. White, A. Patel, H. Varsani, D. Linch, K. Murray, and P. Woo Autologous stem cell transplantation for paediatric-onset polyarteritis nodosa: changes in autoimmune phenotype in the context of reduced diversity of the T- and B-cell repertoires, and evidence for reversion from the CD45RO+ to RA+ phenotype Rheumatology, November 1, 2001; 40(11): 1299 - 1307. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L WEDDERBURN, A PATEL, H VARSANI, P WOO;, S VIGNOLA, F SABATINI, F FALCINI, P PICCO, A BUONCOMPAGNI, A LOY, et al. 1 Basic research Ann Rheum Dis, September 1, 2000; 59(9): 713a - 719. [Full Text]
|
|
|
|
|
|
L. R. Wedderburn Tracking T cells in arthritis Rheumatology, May 1, 2000; 39(5): 458 - 462. [Full Text] [PDF]
|
|