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International Immunology, Vol. 11, No. 2, 259-268, February 1999
© 1999 Japanese Society for Immunology

Core 2-containing O-glycans on CD43 are preferentially expressed in the memory subset of human CD4 T cells

Ryuta Mukasa, Toshio Homma, Takashi Ohtsuki, Osamu Hosono, Akiko Souta, Toshio Kitamura1, Minoru Fukuda3, Sumiko Watanabe2 and Chikao Morimoto

Departments of Clinical Immunology and AIDS Research Center,
1 Hematopoietic Growth Factors, and
2 Molecular and Developmental Biology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan
3 Glycobiology Program, The Burnham Institute, La Jolla Cancer Research Center, 10901 North Torrey Pines Road, La Jolla, CA 92037, USA

Correspondence to: C. Morimoto

Human CD4 T cells can be divided into two functionally distinct subsets: a CD45RO+ memory subset and a CD45RA+ naive subset. In an attempt to identify novel cell surface molecules on these cells, we have developed a mAb, anti-1D4. The antigen defined by anti-1D4 was preferentially expressed on the memory subset of freshly isolated peripheral CD4 T cells and 1D4+ CD4 T cells functionally corresponded to memory T cells. Retrovirus-mediated expression cloning revealed that the 1D4 antigen is human CD43. Transfection of CHO-leu cells, which stably express human CD43, with core 2 ß-1,6-N-acetylglucosaminyltransferase (C2GnT) conferred expression of the 1D4 antigen and mRNA of C2GnT was detected by RT-PCR only in 1D4+ T cells but not in 1D4– T cells, implying that the 1D4 antigen is composed of core 2-containing O-glycans on CD43. Reactivity with anti-1D4 was completely abolished when cells were treated with neuraminidase, while there remained weak binding of anti-T305, a previously described mAb which also reacts with CD43 modified with core 2-containing O-glycans. Moreover, anti-1D4 markedly reacted with NIH-3T3 cells expressing human CD43 and low levels of endogenous C2GnT, whereas anti-T305 reacted slightly. These results indicate that the 1D4 antigen is distinct from the epitope defined by anti-T305 and anti-1D4 is a more sensitive probe to detect core 2-containing O-glycans than anti-T305. Taken together, our results indicate that core 2-containing O-glycans, whose expression can easily be detected with anti-1D4, are preferentially expressed in the CD45RO+ memory subset of CD4 T cells.

Keywords: CD4, CD43, cell surface molecules, core 2 ß-1,6-N-acetylglucosaminyltransferase, O-glycan, selectin ligand

Transmitting editor: T. Saito


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