Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (18)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Mukasa, R.
Right arrow Articles by Morimoto, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mukasa, R.
Right arrow Articles by Morimoto, C.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

International Immunology, Vol. 11, No. 2, 259-268, February 1999
© 1999 Japanese Society for Immunology

Core 2-containing O-glycans on CD43 are preferentially expressed in the memory subset of human CD4 T cells

Ryuta Mukasa, Toshio Homma, Takashi Ohtsuki, Osamu Hosono, Akiko Souta, Toshio Kitamura1, Minoru Fukuda3, Sumiko Watanabe2 and Chikao Morimoto

Departments of Clinical Immunology and AIDS Research Center,
1 Hematopoietic Growth Factors, and
2 Molecular and Developmental Biology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan
3 Glycobiology Program, The Burnham Institute, La Jolla Cancer Research Center, 10901 North Torrey Pines Road, La Jolla, CA 92037, USA

Correspondence to: C. Morimoto

Human CD4 T cells can be divided into two functionally distinct subsets: a CD45RO+ memory subset and a CD45RA+ naive subset. In an attempt to identify novel cell surface molecules on these cells, we have developed a mAb, anti-1D4. The antigen defined by anti-1D4 was preferentially expressed on the memory subset of freshly isolated peripheral CD4 T cells and 1D4+ CD4 T cells functionally corresponded to memory T cells. Retrovirus-mediated expression cloning revealed that the 1D4 antigen is human CD43. Transfection of CHO-leu cells, which stably express human CD43, with core 2 ß-1,6-N-acetylglucosaminyltransferase (C2GnT) conferred expression of the 1D4 antigen and mRNA of C2GnT was detected by RT-PCR only in 1D4+ T cells but not in 1D4– T cells, implying that the 1D4 antigen is composed of core 2-containing O-glycans on CD43. Reactivity with anti-1D4 was completely abolished when cells were treated with neuraminidase, while there remained weak binding of anti-T305, a previously described mAb which also reacts with CD43 modified with core 2-containing O-glycans. Moreover, anti-1D4 markedly reacted with NIH-3T3 cells expressing human CD43 and low levels of endogenous C2GnT, whereas anti-T305 reacted slightly. These results indicate that the 1D4 antigen is distinct from the epitope defined by anti-T305 and anti-1D4 is a more sensitive probe to detect core 2-containing O-glycans than anti-T305. Taken together, our results indicate that core 2-containing O-glycans, whose expression can easily be detected with anti-1D4, are preferentially expressed in the CD45RO+ memory subset of CD4 T cells.

Keywords: CD4, CD43, cell surface molecules, core 2 ß-1,6-N-acetylglucosaminyltransferase, O-glycan, selectin ligand

Transmitting editor: T. Saito


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Immunol.Home page
J. D. Hernandez, J. T. Nguyen, J. He, W. Wang, B. Ardman, J. M. Green, M. Fukuda, and L. G. Baum
Galectin-1 Binds Different CD43 Glycoforms to Cluster CD43 and Regulate T Cell Death
J. Immunol., October 15, 2006; 177(8): 5328 - 5336.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
P. V. Cabrera, M. Amano, J. Mitoma, J. Chan, J. Said, M. Fukuda, and L. G. Baum
Haploinsufficiency of C2GnT-I glycosyltransferase renders T lymphoma cells resistant to cell death
Blood, October 1, 2006; 108(7): 2399 - 2406.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
R. C. Fuhlbrigge, S. L. King, R. Sackstein, and T. S. Kupper
CD43 is a ligand for E-selectin on CLA+ human T cells
Blood, February 15, 2006; 107(4): 1421 - 1426.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. Kyoizumi, T. Ohara, Y. Kusunoki, T. Hayashi, K. Koyama, and N. Tsuyama
Expression Characteristics and Stimulatory Functions of CD43 in Human CD4+ Memory T Cells: Analysis Using a Monoclonal Antibody to CD43 That Has a Novel Lineage Specificity
J. Immunol., June 15, 2004; 172(12): 7246 - 7253.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
B. Walcheck, A. Leppanen, R. D. Cummings, R. N. Knibbs, L. M. Stoolman, S. R. Alexander, P. E. Mattila, and R. P. McEver
The monoclonal antibody CHO-131 binds to a core 2 O-glycan terminated with sialyl-Lewis x, which is a functional glycan ligand for P-selectin
Blood, May 13, 2002; 99(11): 4063 - 4069.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
M. Fukuoka, K. Fukudome, Y. Yamashita, M. Tokushima, K. Miyake, and M. Kimoto
Antiadhesive function of 130-kd glycoform of CD43 expressed in CD4 T-lymphocyte clones and transfectant cell lines
Blood, December 15, 2000; 96(13): 4267 - 4275.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Med.Home page
L. E. Harrington, M. Galvan, L. G. Baum, J. D. Altman, and R. Ahmed
Differentiating between Memory and Effector CD8 T Cells by Altered Expression of Cell Surface O-Glycans
J. Exp. Med., April 3, 2000; 191(7): 1241 - 1246.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.