International Immunology, Vol. 11, No. 2, 217-227,
February 1999
© 1999 Japanese Society for Immunology
Mechanisms of acute inflammatory lung injury induced by abdominal sepsis
1 Department of Surgery, Klinikum rechts der Isar, Technical University, 81675 Munich, Germany
2 Institute for Medical Microbiology, Immunology and Hygiene, Technical University, 81675 Munich, Germany
3 Department of Orthopedic Surgery, Klinikum Grosshadern, Ludwig Maximilian University, 81377 Munich, Germany
Correspondence to: B. Holzmann, Department of Surgery, Klinikum rechts der Isar, Technische Universität, Ismaninger Strasse 22, 81675 Munich, Germany
Sequestration of neutrophils and release of histotoxic mediators are considered important for the development of pathologic alterations of the lung defined as adult respiratory distress syndrome. Mechanisms of inflammatory lung injury caused by abdominal sepsis were investigated using the colon ascendens stent peritonitis (CASP) model that closely mimics the human disease. In the CASP model, a continuous leakage of intraluminal bacteria into the peritoneal cavity is induced by implantation of a stent in the ascending colon, generating a septic focus. In contrast to the cecal ligation and puncture model of peritonitis, survival of mice following CASP surgery is dependent on IFN-
, but independent of tumor necrosis factor (TNF). Here we show that the systemic inflammation induced by CASP surgery results in a rapid and profound increase of lung vascular permeability that was associated with the activation and recruitment of neutrophils to the lung. Activation of circulating granulocytes was characterized by increased production of serine proteinases and reactive oxygen metabolites, as well as elevated expression of cell surface Mac-1. Expression of MIP-2, KC, MIP-1
and E-selectin mRNA in lung was strongly increased within 3 h following CASP surgery, whereas up-regulation of IP-10, MCP-1 and P-selectin was delayed. In contrast, induction of RANTES, LIX, ICAM-1 and VCAM-1 mRNA was weak or not detectable after CASP surgery. Importantly, recruitment of leukocytes to the lung was normal in lipopolysaccharide-resistant mice, and was not affected by antibody neutralization of TNF or the chemokines MIP-2 and KC.
Keywords: cell adhesion molecules, chemokines, IFN-, KC, MIP-2, neutrophil activation, sepsis animal models, tumor necrosis factor-
Transmitting editor: I. L. Weissman
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  T. Secher, V. Vasseur, D. M. Poisson, J. A. Mitchell, F. Q. Cunha, J. C. Alves-Filho, and B. Ryffel Crucial Role of TNF Receptors 1 and 2 in the Control of Polymicrobial Sepsis J. Immunol., June 15, 2009; 182(12): 7855 - 7864. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Wang, T. Rui, M. Yang, F. Valiyeva, and P. R. Kvietys Alveolar Macrophages from Septic Mice Promote Polymorphonuclear Leukocyte Transendothelial Migration via an Endothelial Cell Src Kinase/NADPH Oxidase Pathway J. Immunol., December 15, 2008; 181(12): 8735 - 8744. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Rittirsch, L. M. Hoesel, and P. A. Ward The disconnect between animal models of sepsis and human sepsis J. Leukoc. Biol., January 1, 2007; 81(1): 137 - 143. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-M. Cavaillon and D. Annane Invited review: Compartmentalization of the inflammatory response in sepsis and SIRS Innate Immunity, June 1, 2006; 12(3): 151 - 170. [Abstract] [PDF]
|
|
|
|
 |
|
 J. W. Smith, R. L. Gamelli, S. B. Jones, and R. Shankar Immunologic Responses to Critical Injury and Sepsis J Intensive Care Med, May 1, 2006; 21(3): 160 - 172. [Abstract] [PDF]
|
|
|
|
 |
|
 K. Nakamichi, M. Saiki, M. Sawada, M. Takayama-Ito, Y. Yamamuro, K. Morimoto, and I. Kurane Rabies Virus-Induced Activation of Mitogen-Activated Protein Kinase and NF-{kappa}B Signaling Pathways Regulates Expression of CXC and CC Chemokine Ligands in Microglia J. Virol., September 15, 2005; 79(18): 11801 - 11812. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. W. Saeed, S. Varma, T. Peng, K. J. Tracey, B. Sherry, and C. N. Metz Ethanol Blocks Leukocyte Recruitment and Endothelial Cell Activation In Vivo and In Vitro J. Immunol., November 15, 2004; 173(10): 6376 - 6383. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Nakamichi, S. Inoue, T. Takasaki, K. Morimoto, and I. Kurane Rabies Virus Stimulates Nitric Oxide Production and CXC Chemokine Ligand 10 Expression in Macrophages through Activation of Extracellular Signal-Regulated Kinases 1 and 2 J. Virol., September 1, 2004; 78(17): 9376 - 9388. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. J. Laudes, R.-F. Guo, N. C. Riedemann, C. Speyer, R. Craig, J. V. Sarma, and P. A. Ward Disturbed Homeostasis of Lung Intercellular Adhesion Molecule-1 and Vascular Cell Adhesion Molecule-1 During Sepsis Am. J. Pathol., April 1, 2004; 164(4): 1435 - 1445. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. C. Ulett, N. Ketheesan, T. W. Clair, C. L. McElnea, J. L. Barnes, and R. G. Hirst Analogous Cytokine Responses to Burkholderia pseudomallei Strains Contrasting in Virulence Correlate with Partial Cross-Protection in Immunized Mice Infect. Immun., July 1, 2002; 70(7): 3953 - 3958. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. F. Wang, M. Patel, H. M. Razavi, S. Weicker, M. G. Joseph, D. G. McCormack, and S. Mehta Role of Inducible Nitric Oxide Synthase in Pulmonary Microvascular Protein Leak in Murine Sepsis Am. J. Respir. Crit. Care Med., June 15, 2002; 165(12): 1634 - 1639. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. B. Lentsch, A. Kato, J. T. Saari, and D. A. Schuschke Augmented metalloproteinase activity and acute lung injury in copper-deficient rats Am J Physiol Lung Cell Mol Physiol, August 1, 2001; 281(2): L387 - L393. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. S. Cheng, J. J. Lai, N. W. Lukacs, and S. L. Kunkel Granulocyte-Macrophage Colony Stimulating Factor Up-Regulates CCR1 in Human Neutrophils J. Immunol., January 15, 2001; 166(2): 1178 - 1184. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Weighardt, C. Feterowski, M. Veit, M. Rump, H. Wagner, and B. Holzmann Increased Resistance Against Acute Polymicrobial Sepsis in Mice Challenged with Immunostimulatory CpG Oligodeoxynucleotides Is Related to an Enhanced Innate Effector Cell Response J. Immunol., October 15, 2000; 165(8): 4537 - 4543. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Bauer, C. W. Lush, P. R. Kvietys, J. M. Russell, and D. N. Granger Role of endotoxin in the expression of endothelial selectins after cecal ligation and perforation Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2000; 278(5): R1140 - R1147. [Abstract] [Full Text] [PDF]
|
|