International Immunology, Vol. 11, No. 2, 209-216,
February 1999
© 1999 Japanese Society for Immunology
Characterization of the culture filtrate-specific cytotoxic T lymphocyte response induced by bacillus Calmette-Guérin vaccination in H-2b mice
Laboratory of Mycobacterial Immunology, Pasteur Institute of Brussels, Engelandstraat 642, 1180 Brussels, Belgium
Correspondence to: K. Huygen
Although CD8+ T cells are supposed to play an important role in protective immunity to mycobacteria, cytotoxic T lymphocyte (CTL) responses in this infection remain poorly characterized. We previously demonstrated that bacillus Calmette-Guérin (BCG) immunization of H-2b mice induced CTL able to recognize and kill macrophages incubated with proteins from mycobacterial culture supernatant [culture filtrate (CF) antigens]. In the present study, we have further characterized the lytic activity of these CTL and the processing pathway used for the presentation of CF proteins. We show that they use the degranulation pathway (secretion of perforins and granzymes) as the main lytic mechanism of cytotoxicity and also secrete IFN-
upon incubation with CF-pulsed macrophages. The in vitro presentation of CF proteins to CTL required a processing step inhibited in the cold but insensitive to Brefeldin A. Transporter-associated protein (TAP)-2-deficient RMA-S cells were efficiently recognized and killed by CF-specific CTL, demonstrating the lack of TAP requirement for this presentation. However, recognition of target cells by CTL was abolished when carried out in the presence of chloroquine. These results indicate that a non-classical MHC class I-processing pathway allows the recognition of a CF protein by CTL in BCG-vaccinated H-2b mice.
Keywords: culture filtrate antigens, cytotoxic T lymphocytes, Mycobacterium bovis bacillus Calmette-Guérin, tuberculosis
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  N. Caccamo, S. Meraviglia, C. La Mendola, G. Guggino, F. Dieli, and A. Salerno Phenotypical and Functional Analysis of Memory and Effector Human CD8 T Cells Specific for Mycobacterial Antigens J. Immunol., August 1, 2006; 177(3): 1780 - 1785. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. D'Souza, V. Rosseels, O. Denis, A. Tanghe, N. De Smet, F. Jurion, K. Palfliet, N. Castiglioni, A. Vanonckelen, C. Wheeler, et al. Improved Tuberculosis DNA Vaccines by Formulation in Cationic Lipids Infect. Immun., July 1, 2002; 70(7): 3681 - 3688. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. G. Feng, C. Demangel, A. T. Kamath, M. Macdonald, and W. J. Britton Dendritic cells infected with Mycobacterium bovis bacillus Calmette Guerin activate CD8+ T cells with specificity for a novel mycobacterial epitope Int. Immunol., April 1, 2001; 13(4): 451 - 458. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Smith, R. Brookes, M. R. Klein, A. S. Malin, P. T. Lukey, A. S. King, G. S. Ogg, A. V. S. Hill, and H. M. Dockrell Human CD8+ CTL Specific for the Mycobacterial Major Secreted Antigen 85A J. Immunol., December 15, 2000; 165(12): 7088 - 7095. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Smith, M. R. Klein, A. S. Malin, J. Sillah, K. Huygen, P. Andersen, K. P. W. J. McAdam, and H. M. Dockrell Human CD8+ T Cells Specific for Mycobacterium tuberculosis Secreted Antigens in Tuberculosis Patients and Healthy BCG-Vaccinated Controls in The Gambia Infect. Immun., December 1, 2000; 68(12): 7144 - 7148. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Smith, A. S. Malin, Pauline T., Lukey, S. E. Atkinson, J. Content, K. Huygen, and H. M. Dockrell Characterization of Human Mycobacterium bovis Bacille Calmette-Guerin-Reactive CD8+ T Cells Infect. Immun., October 1, 1999; 67(10): 5223 - 5230. [Abstract] [Full Text] [PDF]
|
|