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International Immunology, Vol. 11, No. 2, 133-141, February 1999
© 1999 Japanese Society for Immunology

Induction of CD8+ T cell-mediated protective immunity against Trypanosoma cruzi

Yasushi Miyahira, Seiki Kobayashi1, Tsutomu Takeuchi1, Tsuneo Kamiyama2, Takeshi Nara, Junko Nakajima-Shimada and Takashi Aoki

Department of Parasitology, Juntendo University School of Medicine, 2-1-1 Hongo, Bunkyo-ku,Tokyo 113-8421, Japan
1 Department of Tropical Medicine and Parasitology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan
2 Department of Veterinary Science, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan

Correspondence to: Y. Miyahira

Trypanosoma cruzi was transformed with the Plasmodium yoelii gene encoding the circum-sporozoite (CS) protein, which contains the well-characterized CD8+ T cell epitope, SYVPSAEQI. In vivo and in vitro assays indicated that cells infected with the transformed T. cruzi could process and present this malaria parasite-derived class I MHC-restricted epitope. Immunization of mice with recombinant influenza and vaccinia viruses expressing the SYVPSAEQI epitope induced a large number of specific CD8+ T cells that strongly suppressed parasitemia and conferred complete protection against the acute T. cruzi lethal infection. CD8+ T cells mediated this immunity as indicated by the unrelenting parasitemia and high mortality observed in immunized mice treated with anti-CD8 antibody. This study demonstrated, for the first time, that vaccination of mice with vectors designed to induce CD8+ T cells is effective against T. cruzi infection.

Keywords: CD8+ T cells, Chagas' disease, protective immunity, recombinant influenza virus, recombinant vaccinia virus, Trypanosoma cruzi

Transmitting editor: R. Nussenzweig


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